蛋白质合成2.pptVIP

The central dogma of molecular biology Gene expression Control of transcription of the lac operon Nucleotide reading frames Agarose gel electrophoretogram of restriction digests Pneumococci Transgenic mice Autoradiograph of Drosophila melanogaster DNA Electron micrograph of a T2 bacteriophage and its DNA Function of the transcription bubble Post-transcriptional processing of eukaryotic mRNAs Replication of duplex DNA in E. coli Replication of duplex DNA in E. coli The 5￠ ? 3￠ exonuclease function of DNA polymerase I Replacement of RNA primers by DNA in lagging strand synthesis The 3￠ ? 5￠ exonuclease function of DNA polymerase I and DNA polymerase III Microinjection of DNA into the pronucleus of a fertilized mouse ovum Use of green fluorescent protein (GFP) as a reporter gene The polymerase chain reaction (PCR) Restriction-fragment length polymorphisms Inheritance of RFLPs according to the rules of Mendelian genetics Electron micrograph of bacteriophage l Electron micrograph of the filamentous bacteriophage M13 Electron micrograph of an inclusion body of the protein prochymosin in an E. coli cell. A degenerate oligonucleotide probe Detection of DNAs containing specific base sequences by the Southern transfer technique Cloning of foreign DNA in l phages Chromosome walking * Stacking of adenine rings in the crystal structure of 9-methyladenine Base stacking associations in aqueous solution are largely stabilized by hydrophobic forces. H-bonding contributes little to the stability of the DNA double helix. Variation of the osmotic coefficient ? with the molal concentrations m of adenosine derivatives in H2O = 1: no association = 0: infinite association p = fRTm = osmotic pressure f measures the degree of association Data are consistent with a successive aggregation model: A + A ? A2 + A ? A3 + A ? …….An where n = at least 5 Schematic representation of strand separation in duplex DNA resulting from heat denaturation heat UV absorbance spectra of native and hea