微生物辅因子平衡的代谢调控.PDFVIP

16 生 物 工 程 学 报 陈修来 等/微生物辅因子平衡的代谢调控 Chinese Journal of Biotechnology /cjbcn January 25, 2017, 33(1): 16−26 DOI: 10.13345/j.cjb.160232 ©2017 Chin J Biotech, All rights reserved ·综 述· 1,2,3 1,2,3 1,2,3 1,2,3 陈修来 ，刘佳 ，罗秋玲 ，刘立明 1 214122 2 214122 3 214122 , , , . . , 2017, 33(1): 16–26. Chen XL, Liu J, Luo QL, et al. Manipulation of cofactor balance in microorganisms. Chin J Biotech, 2017, 33(1): 16–26. 摘 要: 辅因子平衡对于酶制剂、药品和化学品的生产具有重要的作用。为了满足工业化生产的需求，维持 辅因子长期有效的平衡是实现代谢流高效化导向目标代谢产物的必要手段。本文在总结辅因子生理功能的基础 上，从生化工程和代谢工程两方面分析归纳了辅因子的代谢调控策略，并展望了辅因子进一步精深调控的发展 方向。 : 辅因子，NADH ，代谢工程，合成生物学，调控策略 Manipulation of cofactor balance in microorganisms Xiulai Chen1,2,3, Jia Liu1,2,3, Qiuling Luo1,2,3, and Liming Liu1,2,3 1 State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi 214122, Jiangsu, China 2 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi 214122, Jiangsu , China 3 Laboratory of Food Microbial-Manufacturing Engineering , Jiangnan University , Wuxi 214122, Jiangsu, China Abstract: Cofactor balance plays an important role in producing enzymes, pharmaceuticals and chemicals. To meet the Received: June 14, 2016; Accepted: September 23, 2016 Supported by: National Natural Science Foundation of China (No. , the Key Technologies R D Program of Jiangsu Province (No. BE2014652), the Fundamental Research Funds for the Central Universities (No. JUSRP51611A), the Fundamental Research Funds for the Central Universities (No. JUSRP116022), the Research Program for S