松墨天牛和光肩星天牛染色体核型-应用昆虫学报.PDF

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (2 ):299 ～ 307 松墨天牛和光肩星天牛染色体核型* 1  1  2 1 1 1 刘 平 嵇保中 刘曙雯 张 凯 于秀坤 林 晓 (1. 南京林业大学 南京 2 10037 ;2 . 南京市中山陵园管理局 南京 2 100 14 ) 1  1  Karyotypes of Monocham us alternatus and A nop lop hora glabrip ennis. LIU Ping ，JI Bao-Zhong ，LIU Shu-Wen2 ，ZHANG Kai1 ，YU Xiu-Kun1 ，LIN Xiao1 (1. Nanj ing Forestry University ，Nanjing 2 10037 ， China ;2. M anagement Off ice of Sun Yat-sen ’s M ausoleum ，Nanjing 2 100 14 ，China) Abstract The karyotypes of M onochamus alternatus Hope and A nop lop hora glabrip ennis Motschulsky were studied . According to the optimum conditions and the best material for each species of long-horn beetle ，we made and examined the slide specimens of chromosomes. The best material for M . alternatus was testis or ovary of 7 d pupae . The chromosomal number was 2 n = 20. The sex-determining mechanism was Xyp . The chromosome was composed of 5 pairs of large autosomes ，4 pairs of middle autosomes and 1 pair of sex- chromosomes. The sex-chromosomes were middle-sized and situated in the last among all chromosomes. The genome-style was 5 L + 4 M + Xyp . The best material for A . glabrip ennis was testis or ovary in the newly emerged adults ，and its chromosomal number was 2 n = 20. The sex-determining mechanism was Xyp . The chromosome was composed of 6 pairs of large autosomes ，3 pairs of middle autosomes and 1 pair of sex- chromosomes. The sex-chromosomes were middle-sized and situated in the last among all chromosomes. The genome-style was 6 L + 3 M + Xyp . Key words M onochamus alternatus ，A nop lop hora glabrip