携带质粒李斯特菌耐药及重金属抵制特性研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.4 携带质粒李斯特菌耐药及重金属抵制特性研究 张辉，包红朵，周艳，张莉莉，王冉 （江苏省农业科学院 农业部农产品质量安全控制技术与标准重点实验室，江苏省畜禽产品安全性研究重点实验 室，江苏南京 210014 ） 摘要：鉴定单核细胞增生性李斯特菌（Lm ）携带质粒特性，分析携带质粒Lm 对抗生素、重金属镉及苯扎氯铵的敏感性。结果 表明，11株Lm 携带内源性质粒；所有Lm 菌株对阿莫西林、红霉素和利福平均敏感（22/22 ），对头孢拉定100%耐药（22/22 ）；其中 5 株对盐酸万古霉中度耐受，部分菌株对盐酸环丙沙星（17/22 ）、硫酸新霉素（16/22）及盐酸四环素素（9/22 ）具有一定耐受性。耐 镉分析表明，菌株对重金属镉呈现抵制特性（14/22 ），且均为携带质粒菌株；而在苯扎氯铵敏感性检测中，仅3 株携带质粒Lm 菌株 对苯扎氯铵具有抵制性。对Lm 菌株进行噬菌体杀菌检测，结果均能够被噬菌体识别并裂解。综上所述，Lm 分离株耐药性增强，且 对苯扎氯铵和镉有抵制特性，而噬菌体能够杀灭抵制菌株，为控制耐药及抵制性菌株污染提供了新途径。 关键词：单核细胞增生性李斯特菌；抗生素；重金属；噬菌体 文章篇号：1673-9078(2015)4-58-62 DOI: 10.13982/j.mfst.1673-9078.2015.4.010 Characterization of Antibiotic, Heavy Metal, and Benzalkonium Chloride Resistance of Plasmid-carrying Listeria monocytogenes ZHANG Hui, BAO Hong-duo, ZHOU Yan, ZHANG Li-li, WANG Ran (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agricultrue, Key Lab of Animal-derived Food Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) Abstract: The major aims of this study included the characterization of plasmids carried by Listeria monocytogenes (Lm ), and an in-depth analysis of the sensitivity of plasmid-carrying Lm towards antibiotics, the heavy metal cadmium, and benzalkonium chloride. The results of this study showed the presence of endogenous plasmids in 11 Lm strains. All Lm strains displayed average sensitivity to amoxicillin, erythromycin, and rifampicin (22/22), and 100% resistance to cefradine (22/22). Five strains showed intermediate resistance to vancomycin hydrochloride, while some strains were resistant to ciprofloxacin (17/22), neomycinsulphate (16/22), and tetracycl