基于染色质交互数据的基因组组装方法 - 生物技术通报.PDFVIP

生物技术通报 ·特约综述· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 11 :43-50 基于染色质交互数据的基因组组装方法 陶婧芬  谢婷  郑觉非  杨庆勇  张红雨 （华中农业大学信息学院，武汉 430070） 摘 要 ： 伴随着高通量DNA 测序技术的不断推陈出新和价格持续下调，如何将scaffolds 定位于染色体逐渐成为完整参考基 因组获得的关键。高通量染色质构象捕获技术 （High-throughput chromosome conformation capture， 简称Hi-C）的出现为基因组组装 过程中scaffolds 快速锚位提供了契机。相比于传统的基因组组装方法，基于染色质交互组装基因组的策略实验操作简易、实验和 时间成本较低、正确率及分辨率高，在基因组相对复杂的多倍型和高度杂合的物种中有着更大的应用前景。但由于技术本身的限制， 该方法还存在分辨率、背景噪声等问题需要解决，有待进一步改进和提高。 关键词 ： 高通量染色质构象捕获技术；染色质交互 ；基因组组装 ；scaffolds 锚位 DOI ：10.13560/ki.biotech.bull.1985.2015.11.007 Genome Assembly Based on Chromatin Interaction Tao Jingfen Xie Ting Zheng Juefei Yang Qingyong Zhang Hongyu （Huazhong Agricultural University Information Institute ，Wuhan 430070 ） Abstract: With the rapid development of sequencing technology, DNA sequencing is more efficiently and economically and in greater depth than ever before. How to locate the scaffolds into the chromosome becomes the key of getting high-quality genome. High-throughput chromatin conformation capture technique provides a new opportunity for scaffolds anchoring. Compared with traditional method, an assembly method based on chromosome interaction information is simple, low cost experimentation and saving time, and more application is expected in other relative complex polyploidy species. However, because of the limitation of the related technology, the genome assembly based on chromatin interaction still use second-generation technology, there are still many problems to be solved, for example, the resolution and background noise. It is expected to further improvement and enhancement. We still need to make effort to i