严重少精子和无精子的不育患者Y染色体微缺失的检测_宋宁宏.pdfVIP

# 1376# 2006 5 30 86 20 NatlM ed J China, M ay 30, 2006, Vol 86, No120 # # Y 宋宁宏 吴宏飞 张炜 华立新 周作民 冯宁翰 张晶 乔迪 张杰秀 Y 2003 7 ) 2005 3 1 3 ( 80 63) Y 1 3, 21 ( 21/ 1 3, 1 17% ) , 12( 3 9) , 9 ( 3 6)21 , 1 AZFa, 2 AZFb+ AZFc, 2 AZFb, 16 AZFc21 , , 6, 11 AZF , , Y Y; ; ; , () Detection of Y chromosom em icrodelations in patients w ith severe oligozoosperm ia and azoosperm ia * SON G N ing-hong , W UH ong-f ei, ZHANG W ei, H UA L i-x in, ZH OU Zuo-m in, FENG N ing-han, ZHAN G * J ing, QIA O D i, ZHAN G J ie-x iu. D ep a tm ent of U ology, F i st Aff iliated H osp ital, N anj ing M edical Un ive s ity, N anj ing 210029, China Co esp onding autho : W U H ong-f e,i Em ail: w uhongf ei6603@ hotmai.l com Abstract Objective To exp lore the clinical sign ificance of azoosperm ia factor (AZF) region deletion. M ethods Detection of theY-link sequence tagged sites in AZF regionw as conducted by means of 2 m ultiplex polymerase chain reactions am ong 80 patients w ith severe oligozoosperm ia and 63 patientsw ith azoosperm ia, totally 1 3. Results Tw enty-one cases of m icrodeletion w ere found among the 1 3 infertile patients w ith a prevalence of 1 . 7% . PCR analysis show ed that deletion of the portions ofYq in 12 of the 62 idiopath ic infertility patients, 3 being w ith severe oligozoosperm ia and 9 w ith azoosperm ia, and in 9