漯河医专药学系毕业论文格式.docVIP

漯河医学高等专科学校 毕业论文 温脾实肠法组方治疗脾阳不足型泄泻机理的初步研究 学生姓名：赵云 指导老师：朱吾元 所学专业：药学 毕业时间：2014年7月 目录 中文摘要·············································1 英文摘要·············································1 前 言·············································1 实验材料·············································1 实验方法·············································1 实验结果··············································1 实验结论··············································1 实验讨论··············································1 参考文献··············································1 致 谢··············································1 温脾实肠法组方治疗脾阳不足型泄泻机理的初步研究 摘要 目的：观察温脾实肠法组方对脾阳不足泄泻模型大鼠血清中酶活性的影响，探讨温脾实肠颗粒治疗脾阳不足泄泻的机理。方法：取SD大鼠60只，雌雄各半，随即分为正常对照组、模型对照组、附子理中丸组、温脾实肠大剂量组、温脾实肠中剂量组、温脾实肠小剂量组，每组10只。除正常对照组外，其余各组采用苦寒药物加饮食失节法造成大鼠脾阳虚泄泻模型。造模结束后给予相应药物治疗14天，末次给药1h，眼眶静脉丛取血，测定血清中淀粉酶、乳酸脱氢酶、琥珀酸脱氢酶水平。结果：脾阳不足泄泻模型组AMS及LDH水平明显低于正常对照组。经药物治疗后温脾实肠颗粒能明显提高模型大鼠血清中AMS及LDH水平。脾阳不足模型大鼠血清中SDH水平明显高于正常对照组，温脾实肠颗粒可降低模型大鼠血清中SDH水平。结论：温脾实肠颗粒对脾阳不足泄泻的治疗作用与调节血清中消化酶水平有关。 关键词：温脾实肠法；脾阳不足泄泻；AMS；LDH；SDH The Experimental study of wenpishichang formula on rats with spleen-yang deficiency diarrhea Abstract:Objective:Observe the effection of wenpishichang formula on the levels of digestive enzymes in serum of rats with spleen-yang deficiency diarrhea.Methods: SD rats were taken 60, male and female in half, then divided into normal control group,model control group,fuzilizhong Pill group,high dose group,middle dose group and low dose group, 10 rats in each group. The spleen-yang deficiency diarrhea model of rats were induced by dahuang decoction.The AMS,LDH and SDH in serum were determined after the last treatment.Result: wenpishichang formula obviously increased AMS and LDH activity,and also decreased serum SDH activity.Conclusion: Wenpishichang formula can correct the parasecretion of digestive enzymes in rats with spleen-yang deficiency diarrhea. Key words: spleen-yang deficiency diarrhea; wenpishichang formula; digestive enzymes 脾阳不足泄泻为消化系统疾病常见疾病之一，现代医学多将其归属与迁