快速诊断滴虫性阴道炎免疫层析试纸条的初步研制_王雪玲.pdfVIP

DOI :10.13350/j.cjpb.2008.05.018 中 国 病 原 生 物 学 杂 志 2008 5 　3 5 J ourna l of P a thogen B iology 　May 2008, 　Vol.3, No.5 ·379 · · · ＊ 1 1 2 1 1 ＊＊ 王雪玲, 蔡敏, 俞石芳 , 黄慧聪 , 潘长旺 (1., 325035;2.) 【】　　 AP33 AP33 mAb, 、 、(GICA)。 　　, AP33 , 。 , 。　　AP33 20 ng/ml, 4A2 、 4F8 。 , 60 , 90.0%(54/60); 60 , 95.0%(57/60)。92.5%。 　　GICA 、、, , 。 【】　;AP33;; 【】　R531.7　　　【】　A 　　　【】　1673-5234(2008)05-0379-04 [J ourna l of P a thog en B io logy .2008 May;3(5):379-382, 386.] Preliminary development of gold-immunochromatography assay stripe for rapid diagnosis of Trichomonia- sis vaginitis 1 1 2 1 1 WANG Xue-ling , CAI Min , U Shi-fang , HUANG Hui-cong , PAN Chang-wang 　(1.Wenz hou M ed ica l Co llege, Wenz hou 325035, China;2.Th e F irst A f f ilia ted H osp ita l of Wenz hou Med ica l Col leg e) 【Abstract】　Objective　To establish a rapid, simple and effective gold-immunochromatographic assay (GICA)stripe for diagnosis of T richomoniasis vaginitis using the monoclonal antibodies (mAbs)against recombinant adhesion protein 33 (AP33 )of Tr ichomonas vag ina lis .　Methods　Colloidal gold was coupled with mAb against recombinant AP33 to pre- pare a GICA test paper.GICA was then performed by further optimization of the labeling and the conditions were deter- mined.The sixty cases of Trichomoniasis vaginitis patients and sixty cases of other colpitic ones were detected and GICA stripe was then evaluated for its sensitivity and specificity.　Results　The lowest concentration that could be detected was 20 ng/ml.