HPLC法测定多动宁胶囊中盐酸小檗碱的含量剖析.PDFVIP

年 月第 卷第 期 2011 1 8 1 ·药品鉴定 · HPLC 法测定多动宁胶囊中盐酸小檗碱的含量 陈云松 （丹东市中心医院，辽宁丹东 118002） 摘要 目的：建立高效液相法测定多动宁胶囊中盐酸小檗碱含量的方法。 方法：采用 钻石（ [ ] Diamonsil-C TM 4.6 mm× 18 ， ）色谱柱；流动相：甲醇 水（ ）；检测波长： ；流速： 。 结果：盐酸小檗碱在 250 mm 5 μm - 40∶60 345 nm 1.0 ml/min 0.050 3～ 范围内与峰面积呈良好的线性关系（ ），平均回收率为 ， （ ）。 结论：本法简 0.804 8 μg r=0.999 8 98.33% RSD=0.63% n=9 便、准确、重现性好，可用于多动宁胶囊中盐酸小檗碱的质量控制。 关键词 多动宁胶囊；盐酸小檗碱； 法；含量测定 [ ] HPLC [中图分类号] R927.2 [文献标识码] A [文章编号] 1673-7210 （2011 ）01 （a ）-059-03 Content determination of Berberine Hydrochloride in Duodongning Capsules by HPLC CHEN Yunsong (The Central Hospital in Dandong City, Dandong 118002, China) [Abstract] Objective: To establish a method for the content determination of Berberine Hydrochloride in Duodongning Capsules by HPLC. Methods: The Diamonsil-C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of methanol and water (40∶60), the flow rate was 1.0 ml/min and the detection wavelength was at 345 nm. Results: The lin- ear ranges of Berberine Hydrochloride was at 0.050 3-0.804 8 μg (r =0.999 8), the average recovery was 98.33% with a RSD of 0.63% (n=9). Conclusion: The method is fast, reliable, accurate and can be used in the quality contro