链格孢菌酯酶同工酶分析及其分类学意义.pdfVIP

第5卷第4期 菌 物 研 究 Vo1．5 No．4 2OO7年l2月 Journal of Fungal Research Dec．20ar7 链格孢菌酯酶同工酶分析及其分类学意义 孙 霞 ， ，王 波3，张天宇 (1．山东农业大学植物病理系，泰安271018；2．莱芜职业技术学院信息工程系，莱芜271100； 3．莱芜出入境检验检疫局，莱芜271100) 摘 要：分析了2o株链格孢菌的酯酶同工酶聚丙烯酰胺凝胶电泳图谱，并将酶谱进行聚类分析。结果发现： 2o株链格孢菌在37％的相似系数处明显分为大孢子组和小孢子组；大孢子组在52~A的招似系数处分为6组， 每组代表1个种，小孢子组在较高的相似性水平上分组与形态分组结果基本一致。本试验结果还表明￡酯酶 同工酶电泳方法简单易行，能准确反映种间的微小差异，适用于链格孢属真菌的种级分类，可作为传统形态学 分类的一个重要辅助手段。 关键词：链格孢属；酯酶同工酶；聚类分析；分类 中图分类号：Q949．331 文献标识码：A 文章编号：1672—3538(2007)04—0229—04 Esterase Isozyme Analysis of Alternaria and Its Taxonomic Significance SUN Xia’-一，WANG Boa，ZHANG Tian—yu’ (1．Department of Plant Pathology，Shandong Agricultural University，Taian 271018，China；2．De— partment ofInformation Engineering，Laiwu College of Vocational Technology，l~dwu 271100，China； 3．Laiwu Entry-Exit Inspection and Quarantine Bureau，Laiwu 271100，China) Abstract：Esterase isozyme zymogram polymorphisms of 20 strains in Altermtria were investigated using polyacrylamide ge1 electrophoresis．The results showed that the strains were evidently divided into large— and sma1]一spored species at 37％ similarity level based on cluster analysis of EST isozyme patterns．and at 52％ similarity level，the large·spored species could be divided into six clusters which represented a species respectively．The small—spored species were also found basically parallel to the morphological groupings at high similarity leve1．The result revealed that the electrophoretic technique could be used to classify Alternaria on specific rank，and it is a useful assistant tool in traditional taxonomic methods． Key words：Alternaria；esterase isozyme；cluster analysis；taxonomy 链格孢属真菌是半知菌暗色丝孢菌中重要的