DNA稳定同位素探针技术在宏基因组学中的应用及前景创新.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2011, April 25; 27(4): 539−545 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2011 CJB, All rights reserved. 综 述 DNA 稳定同位素探针技术在宏基因组学中的应用及 前景 刘威，魏晓，袁静，黄留玉 军事医学科学院疾病预防控制所，北京 100071 摘 要: DNA 稳定同位素探针 (DNA-SIP) 是一种新兴的技术，通过将同位素稳定结合到特定的底物来确定环境中微生 物的作用。DNA-SIP 与宏基因组学结合可以让某些微生物的特性与其特殊新陈代谢联系在一起，不仅可以从宏基因组 库里检测到低含量的微生物，而且加速了对新的酶类和其他生物活性物质的发现。以下总结了 SIP-宏基因组学技术的 原理、应用及研究进展，并讨论了其在环境微生物学和生物技术的应用前景。 关键词: 宏基因组学，同位素探针，微生态，DNA-SIP Applications and perspectives of DNA stable-isotope probing in metagenomics: a review Wei Liu, Xiao Wei, Jing Yuan, and Liuyu Huang Institute of Disease Control and Prevention , Academy of Military Medical Sciences , Beijing 100071, China Abstract: DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology. Keywords: metagenomics, stable-isotope probing, microecosystem, DNA-SIP 基因序列的研究大致经历了 3 个过程，早期主 长 DNA 片段和整个群落基因序列的分析，随着高 要关注对 16S rRNA 基因的分析[1] ，后来开始关注 通量基因测序技术的发展，现