神经干细胞-2013142.pptVIP

神经干细胞的研究趋势 进一步研究神经干细胞生物学特征以及分离、纯化和扩增的条件 人类神经干细胞在脑内的定位 怎样在原位诱导神经干细胞增殖分化 Thank you ! * * * * * * * * 1992 年 ,Reynolds等首次用 Neurosphere法成功地从成年小鼠纹状体分离出神经干细胞(neural stem cells ,NSCs). * * * neural cells, in the damaged area. ROI analysis with repeated measurement statistics showed mean tracer uptake in the damaged territory increased significantly in both groups, and the mean tracer uptake in implantation group was significantly higher than control groups. The mean tracer uptake in the damaged areas among the control group increased from 72.1±12.5% to 125±9.4% at 12-month follow-up, whereas it increased strongly from 71.4±7.9% to 161.3±4.6% in implantation group (Fig. 7 A-D). In the patients with 2-year follow-up, the difference of the FDG-PET data between pre-implantation and post-implantation at different intervals were analyzed with SPM method. Using * * * * * * * Approaches to the therapeutic use of stem cells (A) Exogenous stem cells may be isolated and expanded in vitro in preparation for grafting into the brain by stereotaxic injection. The exogenous stem cells could come from a variety of sources including embryonic stem cells or tissue stem cells. Tissue stem cells could be isolated from a variety of somatic tissues, from bone marrow, or from extra-embryonic tissue, such as the umbilical cord. Induced pluripotent stem cells, derived from somatic cells that have been reprogrammed to pluripotency, may be used in research studies for the benefit they offer for understanding disease pathology. Thus, by introducing diseased stem cells to healthy brain or healthy stem cells to diseased brain, induced pluripotent stem cells could be useful for discriminating the cell autonomous deficiencies of diseased neurons relative to the environmental support offered by the diseased brain. (B) Cultured stem cells could also be genetically modified ex vivo prior to stereotaxic injection into the brain. This approach could be useful for expressing cell autonomous signals within g