环氧合酶2启动子甲基化在食管鳞癌中的作用 - 首都医科大学学报.PDFVIP

2010 6 Jun. 2010 3 1 3 Journal of C apitalM ed ical U niversity V o l. 31 N o. 3 [ do:i 10. 3969 / j. issn. 2010. 03. 008] 环氧合酶2 启动子甲基化在食管鳞癌中的作用 * 王青釭 朱圣韬 张澍田 ( , ! 2( cyc looxygenase2, COX2 COX2 2 COX2 COX 2TE1, RT - PCR m RNA , DNA COX2 5 , COX2 COX2m RNA M TT EL ISA E2( prostag land in E2, PGE2 TE1 COX2 , , 58. 3% 5, COX2 30% , , 16. 2% , 5 COX2, 4 8. 63% , PG E2 553. 96 pg /mL 647. 54 pg /mL 2 , 2, 2 , ! ; ; ; ! R 735. 1 E ffects of P rom oter M ethy ation of Cyc ooxygenase2 on Lower Expressed E sophagea Squam ou s C e L in e * WANG Q inggang, ZHU Shengtao, ZHANG Shutian (D ep artm ent of Gastroentero logy, B e ij ing Fr iendsh ip H osp ita,l Cap ital M ed ical Un iv ersity, B e ij ing D ig es tive D isease C enter ABSTRACT! Objective T o determ ine the relationship betw een cyclooxygenase2 ( COX2 expression and its promo ter m ethy lation statu s in low er expressed COX2 esophageal squam ou s cell carc inoma ( ESCC , as w e ll as to investigate the sign if icance of comb ining dem ethy la ting agent w ith se lec tive COX2 inh ibitor in depression o f ESCC. M ethods ESCC ce ll line TE1w as used in this study, and 5 azadeoxycytid ine( 5azaDC and n mi esu lide w ere added into the cultured TE1 cell line. Prom oter m ethy lation statu s o f cyclooxygenase2 w as exam ined by b isu lfate sequencing analysis. RTPCR w as u sed to de term ine the expression of COX2 m RNA. Ce lls pro life ration of ESCC w as tested