GPR30在小鼠发情周期卵巢中的分布和表达.PDFVIP

安徽农业大学学报, 2012, 39(6): 863-866 Journal of Anhui Agricultural University 网络出版时间：2012-10-26 14:59:27 [URL] /kcms/detail/34.1162.S1459.025.html GPR30 在小鼠发情周期卵巢中的分布和表达 耿阳雪，蒋振兴，熊东升，代盈盈，王文丽，赵慧英* (西北农林科技大学动物医学院，杨凌 712100) 摘 要：为了获得 G 蛋白偶联受体 30 （GPR30 ）在小鼠发情周期卵巢中的分布和表达规律，分别运用免疫组 织化学 SP 法和 RT-PCR 法，对小鼠发情前期、发情期、发情后期和间情期卵巢中 GPR30 分布及 GPR30 mRNA 的 表达进行研究。结果显示： GPR30 免疫反应阳性产物分布于小鼠卵巢各级卵泡的颗粒细胞、卵泡膜细胞、卵母细 胞以及黄体细胞和间质细胞的细胞膜和胞浆中，以次级卵泡和成熟卵泡的颗粒细胞中分布最多、着色最深；GPR30 的相对表达量在小鼠发情周期卵巢中以间情期最低且极显著低于其他 3 个时期（P ＜0.01 ），发情期最高但与发情前 期和发情后期无显著差异（P ＞0.05 ）。发情周期卵巢中 GPR30 mRNA 的表达变化规律与 GPR30 的表达基本一致， 其中发情期最高且极显著高于其他时期（P0.01 ），间情期最低且极显著低于其他时期（P0.01 ）。GPR30 在小鼠 卵巢中的分布和表达量变化与卵泡发育过程以及发情周期雌激素变化规律基本一致，提示其介导了雌激素对发情周 期卵泡发育和卵母细胞成熟的调节。 关键词：GPR30；小鼠；卵巢 中图分类号：Q954.62; S865.13 文献标识码：A 文章编号：1672−352X (2012)06−0863−04 The distribution and expression of GPR30 in mice ovary during the estrous cycle GENG Yang-xue, JIANG Zhen-xing, XIONG Dong-sheng, DAI Ying-ying, WANG Wen-li, ZHAO Hui-ying (College of veterinary medicine, Northwest A ﹠F University, Yangling 712100) Abstract: To obtain the distribution and change of GPR30 in the mouse ovary during the estrous cycle, im- munochemistry SP and RT-PCR methods were used respectively to study the expression of GPR30 and GPR30 mRNA in the mouse ovary during different stages of the estrous cycle. As a result, positive staining of GPR30 was widely located in the granular cells, theca interna cells of the follicles at different levels, oocytes, the luteal cells and interstitial cells, while the deepest staining of GPR30 was found in secondary follicles and mature follicles’s granular cells. The immunoreactivity positive substance was mainly distributed in the cell membrane and the cy- toplasm. The relative expression levels of GPR30 reached the lowest in the