3株巨型艾美耳球虫免疫原性差异株.PDFVIP

安徽农业大学学报, 2011, 38(1): 48-50 Journal of Anhui Agricultural University [DOI]CNKI: 34－1162/S1412.001 3株巨型艾美耳球虫免疫原性差异株 未孢子化卵囊蛋白的 SDS分析 1 2 1 1 李文超 ，陶建平 ，顾有方 ，陈会良 (1.安徽科技学院动物科学院，凤阳 233100 ；2.扬州大学兽医学院，扬州 225009) 摘 要：利用聚丙烯酰胺凝胶电泳技术（SDS）比较分析了3 株巨型艾美耳球虫免疫原性差异株未孢子 化卵囊可溶性蛋白。结果显示，尽管所用的3 株巨型艾美耳球虫在免疫原性上存在不同程度的差异，但 3 株巨型 艾美耳球虫未孢子化卵囊可溶性蛋白的SDS电泳图谱相似，至少有8 条带，各带主次分明，其中有2 条明 显主带，分子量分别为42 000 和39 000 ，还有3 条较明显主带，分子量为36 000、26 000 和22 900 ，其他可见带 分别为109 800、86 900 和62 800。结果表明SDS电泳不能检测出E.maxima 免疫原性差异株未孢子化卵囊 可溶性蛋白的差异。 关键词：巨型艾美耳球虫；未孢子化卵囊；免疫原性差异株 中图分类号：S852.723 文献标识码：A 文章编号：1672−352X (2011)01−0048−03 SDSanalysis of unsporulated oocyst proteins of three different immunogenic strains of Eimeria maxima 1 2 1 1 LI Wen-chao , TAO Jian-ping , GU You-fang , CHEN Hui-liang (1. College of Animal Science, Anhui Science and Technology niversity, Fengyang 233100 ； 2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009) Abstract: The soluble protein profiles of unsporulated oocysts among the three geographical strains of Eimeria maxima were compared by SDS(sodium dodecyl sulfate-polyacrylamide gel)electrophoresis tech- nique. The results showed that the band profile from each of the strains were similar, although there were different in the immunogenicity of the three Eimeria maxima to some degree. There were at least eight protein bands in unsporulated oocyst, including two distinct proteins with 42 000 and 39 000, three relative distinct proteins with 36 000, 26 000, 22 900, and less-distinct proteins