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Supporting Information © Wiley-VCH 2007 69451 Weinheim, Germany 1 Polymer Vesicles Containing Small Vesicles within Interior Aqueous Compartments and pH-Responsive Transmembrane Channels 1, 1 1 1 Hsin-Cheng Chiu *, Yue-Wen Lin , Yi-Fong Huang , Chih-Kai Chuang , and Chorng-Shyan Chern2 1Department of Chemical Engineering, National Chung Hsing University, Taichung 401, Taiwan 2 Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan *To whom correspondence should be addressed: E-mail: hcchiu@dragon.nchu.edu.tw Experimental Section Synthesis of Copolymers of Acrylic Acid (AAc) and Distearin Acrylate (DSA): N-Acryloxysuccinimide (NAS) and poly(NAS) were synthesized as described previously [1,2]. In brief, NAS was prepared by esterification of N-hydroxysuccinimide with acryloyl chloride and the structure was verified by 1H NMR, FTIR and elemental analysis. Free radical polymerization of NAS was performed in DMF using 2,2’-azobis(isobutyronitrile) as the initiator at 60 oC for 15 h with stirring under a nitrogen atmosphere. The copolymers, poly(AAc-co-DSA), were prepared first by partial transesterification of poly(NAS) with distearin in DMF with a theoretical total solid content of 10% (w/v) at 60 oC for 120 h. The reagent 4-dimethyl-aminopyridine (DMAP) was used as the catalyst. This was followed by thorough hydrolysis of the remaining NAS residues into AAc units in DMF/H2O (2/1 (v/v)) solution at room temperature for 7 days. The crude product was dialyzed (Spectra/Por MWCO 6000~8000) against DMF and deionized water se