水稻螺旋生长突变体hk759的生理特性及遗传分析研究.pdfVIP

量增高，hk759 的螺旋程度加强，螺旋的圈数增多，螺旋的直径越来越小，螺旋的程 度越来越密集；当IAA含量达到8mg·L-1 时，螺旋圈数为2.72，而螺旋的直径仅有 3.42cm，分别为空白对照组的2.06倍和82.3%。 关键词： 水稻，螺旋突变体，生理生化，内源激素 II Abstract Through physical and chemical mutagenesis generated loss-of-function mutant and obtainedfunctionmutantgeneillustratedthefunctionsofgenes.Itwasoneimportantway tostudyfunctionalgenomics. We obtained a rice mutant hk759 obtained from wuyunjing7 by heavy ion irradiation .Onthe study wemainlyresearchedtheagronomiccharacters,physiological ， andbiochemical,theenvironmentfactorsandregulationmechanismofhelical formedand geneticanalysis.Theexperimentconclusionsasfollows: 1Studyoncharacteristicsofmutanthk759 1.1 The helical growth characteristics of mutant expressed during the whole plant growth and the whole plant organs. The mutant showed helical growth at seeding stage. The mutant showed anticlockwise helical growth in the root, leaves, stem and grains at mature stage.At mature stage, the mutant also showed tiller angle increased, plant type loosed,dwarf, leafhalfrolled andparthelical.Therootlengthandnumber ofmutant also significantlyincreased.Thehelicaldegreewassignificantlyenhanced.Thehulldehiscence ratewashigh. 1.2Thehelicalformwasconductedbyunevengrowthofcells.Weobserveditfromthe longitudinal sectionofhelical stemsbyparaffin section.Inthenormalpart ofmutant,the number and length andwidth ratio between left andright epidermal cellswere the same. But inthe helical part ofmutant,the inner epidermal cells length ofthehelical part were smallerthan the outside epidermal cells length ofthe helical part. Onlywere 88%ofthe outside epidermal cells of the helical part. The inner epidermal cells width of the helical partwas 1.08timeswiderthantheoutside. 1.3Genetic analysis showedthat the mutant was controlledby a singlerecessive gene anditsgeneticstability.Themutantgenewasm