连续性血液净化对严重脓毒症患者Toll样受体及炎症介质的影响.pdfVIP

• 331 • 一一生国主盘!(主主主主医主主主 2010年4 月第5 岳重旦旦 ·论著· 连续性血液净化对严重服毒症患者Toll 样受体及 炎症介质的影晌 郑建鹏茅尧生曹森英 【摘要】目的探讨连续性血液净化(CBP)对严重服毒症患者Toll 样，受体及炎症介质的影响。 方法 30 例严重服毒症患者随机分成常规治疗组和CBP治疗组，每组 15 例:另选 15 名健康志愿者为 对照组。在治疗的不同时间点，检测单核细胞Toll 样受体-2(fLR2)和TLR4 的蛋白表达及血清 TNF-a 、 IL-6 、 IL-1O的浓度。结果严重服毒症患者TLR2/4 的蛋白表达及TNF-α 、 IL-6 、 IL-1O的 浓度水平，均明显高于健康对照组(均PO.05)o CBP 可下调TLR2/4 的蛋们表达及T川、-α 、 IL-6 的浓 度水平(P0.05)。结论 CBP通过下调TLR2I4 的蛋内表达，抑制l过于亢进的非特异性免疫反应;通 过降低血清炎症介质的浓度，使促炎和抗炎反应达到相对平衡。 Z关键词】连续性血液净化;严重服毒症; Toll 样受体 EtTect of continuous blood purification on toll-like receptor and inflammatory mediator in patients with severe sepsis ZHENGJían-peng, MAO Yao-sheng, CAO Míao-yíng. Wenzhou Medíc a1 CoJlege, Wenzhou 325035, Zhejiang China Correspondíng author: MA 0 Yao-sheng, Emaíl: mys5959@163.com Abstract Objective To explore the effect of continuous blood puri行cation (CBP) on toll-like receptor and inflamrnatory rnediator in patients with severe sepsis. Methods A total of thirty patients WÎth severe sepsis were enrolled randornly in two groups: fifteen in common therapy group and the other fifteen in CBP therapy group. Another fifteen healthy volunteers served as control. At different time points of therapy , the protein expression of to Il-like receptor-2σLR2)， TLR4 on monocyte was investigated by flowcytometry and the serum concentrations of turnor necrosis factor-α (fNF-叫， interleukin-6 (IL-6) , IL- 1O were measured by enzyme linked immunosorbent assay (E LlSA). Results Patients with seve陀 sepsis were characterized by an increased proteín expr，臼sion of TLR2, TLR4 on monocyte and an elevated serum concentration of TNF-α， IL-6 and IL-IO compared 10 controls (P0.05). CBP down-regulated the protein expres