畜禽血常规检验1-1104.pptVIP

一、血红蛋白测定（Hb）：沙利氏法 1、原理： 盐酸与血红蛋白结合形成盐酸高铁血红蛋白而变为褐色，通过与标准比色柱比色，可测得血红蛋白的含量（g/L）。 2、试剂与设备：① 1%盐酸（白细胞稀释液）；② 沙利氏血红蛋白计。 3、方法： 将1%盐酸注入血红蛋白测定管内的刻度20%处。用沙利氏吸管精确吸取20微升血样，用脱脂棉擦去外壁附着血液后将其吹入测定管内，并反复吸吹数次，用小玻棒摇匀，静置5～10min。然后用蒸馏水稀释至测定管内颜色与标准比色柱颜色一致为止。读取液体凹面处刻度，即为100ml血液所含血红蛋白百分数或克数。 4、注意事项 ① 该法测定Hb的读数单位为g/100ml血液。 ② 用Hb吸管吸取血样前后均应及时将Hb吸管用三样瓶进行冲洗干净。 冲洗顺序为：水 酒精 乙醚 5、正常参考值 各种动物血红蛋白正常值在90～120g/L。 6．临床意义 ① 血红蛋白增多：主要是见于脱水，血红蛋白相对增加。也见于真性红细胞增多症，是一种原因不明的骨髓增生性疾病，目前认为是多能干细胞受累所致。其特点是红细胞持续性显著增多，全身总血量也增加，见于马、牛、犬和猫。 ② 血红蛋白量减少：主要见于各种贫血。 Diagram Concept Add Your Text Text Text Text Text Text Text Diagram Add Your Text Add Your Text Add Your Text Add Your Text Add Your Text Add Your Text Add Your Text Add Your Text Diagram Text Text Text Title Text Text Text Add Your Text Diagram Add Your Title Add Your Title Add Your Title ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. Diagram Add Your Title Text Text Text Text Cycle Diagram Concept B E C D A Add Your Text Add Your Text Add Your Text Add Your Text Add Your Text Diagram 1 ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. 2 ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. 3 ThemeGallery is a Design Digital Content Contents mall developed by Guild Design Inc. Diagram Your Text Your Text Your Text Your Text Your Text Your Text Your Text Your Text 2001 2002 2003 2004 Progress Diagram Phase 1 Phase 2 Phase 3 Block Diagram TEXT TEXT TEXT TEXT TEXT TEXT TEXT TEXT Table Title Title Title Title Title Title O O O O O Title O O O O O Title O O O O O Title O O O O O Title O O O O O Title O X O X O Text1 Text2 Text3 Text4 Text5 3-D Pie Chart Block Diagram Add Your Text Add Your Text concept Concept Concept Concept 西南大学荣昌校区