稻瘟病抗性基因的分离和鉴定.pdfVIP

Plant Molecular Biology 54: 99–109, 2004. 99 © 2004 Kluwer Academic Publishers. Printed in the Netherlands. Isolation and identification of a gene in response to rice blast disease in rice 1 1 1 2 1 Xianwu Zheng , Xuewei Chen , Xiaohong Zhang , Zhongzhuan Lin , Junjun Shang , Jichen Xu1, Wenxue Zhai1,∗ and Lihuang Zhu1,∗ 1 ∗ Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China ( author for correspondence; e-mail lhzhu@); 2Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing, 100085, China Received 21 October 2003; accepted in revised form 8 January 2004 Key words: bulked segregant analysis, cDNA-AFLP, rice blast disease, TDFs Abstract We combined cDNA amplified fragment length polymorphism (cDNA-AFLP) with bulked segregant analysis (BSA) to detect genes that control rice blast (Magnaporthe grisea) resistance in a double-haploid (DH) population derived from a cross between a blast-resistant variety, Zhai Ye Qing8 (ZYQ8), and a blast-susceptible variety, Jin Xi17 (JX17). In cDNA-AFLP analysis between a blast resistance (R) pool and a blast susceptibility (S) pool from the DH population, 12 transcript-derived fragments (TDFs) that were present in only one of the two pools were detected, 8 of which were from the R pool and 4 from the S pool. Mapping analysis of these TDFs by using the DH mapping population showed that five of them, R1, R8, S9, S16 and S17, were located on chromosome 1. Sequence comparison and allelic analysis showed thatR1/S16 and R8/S9 were two pairs of allelic genes. The full- length cDNA seq