微生物限制-直接接种方法以及菌落计数(国外英文资料).docVIP

微生物限度-直接接种方法以及菌落计数 The total number of colonies Total number of colonies is refers to under certain conditions, such as aerobic condition, nutrition condition, pH, temperature and time, etc.) per gram (per milliliter) test samples grown by the total number of bacterial colonies. As stipulated in the national standard method, namely under the aerobic condition, 37 ℃ 48 h, can grow in the common nutrient AGAR plate on the total number of bacterial colonies, so the micro aerobic bacteria, anaerobic or have special nutritional requirements and the mesophilic bacteria, due to the existing conditions cannot satisfy their physiological needs, it is difficult to reproduce. So it doesnt mean the all the total number of bacteria in the actual total number of colonies, the total number of colonies cannot distinguish between the types of bacteria, so, sometimes called bacterial number, number of aerobe, etc. directory The total number of colonies was introduced Inspection methods Description (1) processing and dilution of the sample: (2) pour culture (3) counting and reporting The total number of colonies was introduced Colony is refers to the bacteria to grow on the solid culture medium and can be formed by the naked eye identification of growth, it is composed of tens of thousands of the same bacteria collection. When the sample was diluted to a certain degree, mixed with culture medium, in a certain culture conditions, each can grow bacterial cells can form a visible colonies on the tablet. Determination of the total number of colonies is used to determine the extent of the food contaminated by bacteria and health quality, which reflects the food in the process of production is in line with the hygiene requirements, so that to be test sample make proper hygiene evaluation. The total number of colonies is partly a sign of the quality of food hygiene. Inspection methods Determination of the total number of colonies, generally will be made into several different test sample to