利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA.pdfVIP

2017 44 3 673678 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j mi RN A mi RNA miRNA miRNA RNA miRNA P miRNA PCR miRNA miRNA miRb miR miRNA PCR miRNA miRNA miRNA S A Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray LI Sheng XU Yanan NIU Xi RAN Xue in q Institute o A robio En ineering Guiz hou Universit Gui ang China f g g y y Faculty of A nim al Sc ience Guiz hou Univ e rs ity Guiy ang China Abstract T o determine the roles of miRNA in the developmental stages of Xiang pig w e investi gated the miRN A ex pression patterns in diff erent months old of Xiang pig s T he total RN A f rom and months old of Xiang pig s w ere collected separately T hen the to tal RNA w ere hy bridized w ith the miRN A microarray and the threshold f oldchange P w ere used to identify the diff erent ex pression miRN A T he quantitative Realtime PCR w as perf ormed to verify the mi croarray result Compared to the months old group there w ere miRNA dow n regulated and miRN A w ere up regulated in the months old group T he low est dow n regulated miRNA w as miRb w hereas the highest up regulated one w as miR T he result of quantitative Realtime PCR consistented w ith the microarray result T he result suggested that some of the dy sregulated ex pression of miRN A might participate in the lipid metabolism in hepatic cells Key