一株产角蛋白酶的铜绿假单胞菌的分离鉴定.pdfVIP

2017 44 3 920927 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j h GenBank BAM A Pes udomonas ae rug inos a B h U mL Pes udomonas ae rug inos a B X A Isolation and Identification of A Keratinase roducing Strain of Pseudomonas aeruginosa B12 p HUANG Miaorong Z HONG Chuhong REN Guangcai LIU Chuan ao YE Junxian CHEN Ruiai g K ey L aboratory of B iotechnology and D rug M anuf acture f or A nimal Ep idemic Prev ention M inistr o A riculture Zhao ing China Colle e o Veterinar Medicine South China y f g q g f y A gricultural Univ ersity Guangz hou China Abstract A new f eatherdegrading bacterium w as isolated f rom soil samples colected f rom pile up f eather places in this study T he transparent circle w as produced af ter the strain w as cultured on the milk medium f or h the colony shape of the strain w as irregular rough surf ace edge blur and could produce g reen f luorescent pigment It w as g ram negative short rod no spore and capsule and had a f lagellum scanning by electron microscope Biochemical test show ed that the isolate could hy drolyze milk and gelatin utilize citrate w ith keratinase activity and nitrate reduction reac tion positive Phylogenetic tree analy sis show ed that the isolate w as most closely related to Pse udomonas ae r ug inos a strain GenBank accession No BA M ACombined w ith the above result s the isolated strain w as identified as Pseudomonas ae r ug inos a B Wh