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Journal of Chromatography A, 1361 (2014) 169–177 Contents lists available at ScienceDirect Journal of Chromatography A j o u r n a l h o me p a ge : w w w. elsev /locate/chro ma Impact of reversed phase column pairs in comprehensive two-dimensional liquid chromatography Robert C. Allen, Brian B. Barnes ∗ , Imad A. Haidar Ahmad, Marcelo R. Filgueira, Peter W. Carr University of Minnesota, Department of Chemistry, Smith and Kolthoff Halls, 207 Pleasant Street SE, Minneapolis, MN 55455, USA a r t i c l e i n f o a b s t r a c t Article history: A major issue in optimizing the resolving power of two-dimensional chromatographic separations is the Received 8 April 2014 choice of the two phases so as to maximize the distribution of the analytes over the separation space. In Received in revised form 2 August 2014 this work, we studied the choice of appropriate reversed phases to use in on-line comprehensive two- Accepted 5 August 2014 dimensional liquid chromatography (LC ×LC). A set of four chemically different conventional bonded Available online 13 August 2014 reversed phases was used in the first dimension. The second dimension column was either a conventional bonded C18 phase or a carbon-clad phase (CCP). The LC ×LC chromatograms and contour plots were all Keywords: rather similar indicating that the selectivities of the two phases were also similar regardless of the reverse Comprehensive two dimensional liquid