基于凝血活性和星点设计―效应面法的黄槐复方提取工艺研究.docVIP

　　基于凝血活性和星点设计―效应面法的黄槐复方提取工艺研究 [摘要]?\用纤维蛋白原平板法，以凝血酶为参照，建立体外凝血活性测定的方法，以体外凝血活性为指标，筛选黄槐复方提取路线，并在单因素试验基础上，以黄芩苷、芦丁提取转移率和浸膏得率的综合评分值为因变量，乙醇浓度、溶剂用量、提取时间为影响因素，利用星点设计3因素5水平试验，进行二次多项式拟合，通过效应面法优选黄槐复方提取工艺，并测定其凝血活性。结果表明凝血酶浓度在04～16 U?mL-1与沉淀圈面积呈良好线性关系，r=0997 5；平均加样回收率为1038%，RSD 47%；黄槐合提浸膏的沉淀圈面积3881 mm2，比分提浸膏沉淀圈面积大；黄槐浸膏的日用量的活性8428 U。黄槐复方的最佳提取工艺为加6倍量40%乙醇，提取3次，每次2 h，综合评分值预测值为9426，验证值为8834，与预测值偏差为628%，最佳提取工艺制得的中间体凝血活性较好。所建立的方法可以简便、快速、准确地测定黄槐复方的凝血活性，可用于后续工艺的筛选以及质量控制。 中国 1/vie 　　[关键词]黄槐复方； 凝血活性； 纤维蛋白原平板法； 星点设计效应面法 　　Optimization of extraction technology from pound Huanghuai 　　based on coagulation activity and central posite design 　　response surface methodology 　　LI Pengcheng1， LIU Tao2*， ei2， LIU Cui2 　　（1 Sichuan Industrial Institute of Antibiotics， Chengdu University， Chengdu 610052， China； 　　2 College of Pharmacy and Biological Engineerin， Chengdu University， Chengdu 610106， China） 　　[Abstract]To establish a method for the determination of coagulation activity in vitro by using fibrinogen plate method The extraction route of pound Huanghuai ized by selecting thrombin as the reference substance The prehensive score of extract yield and the extraction transfer rate of baicalin and rutin e and extraction time as the influence factors in central posite design for quadratic fitting， and the extraction process of pound Huanghuai ized by using response surface methodology The results of thrombin concentration and precipitation zone area shoL-1， r=0997 5 The average recovery rate m2， ount of pound Huanghuai extract um extraction technology es， the liquidsolid ratio 6∶1， and extraction time 2 h The predicted value of prehensive score easured value ediate obtained by optimal extraction process ethod established in this paper ple， fast and accurate for determination of coagulation activity of pound Huanhuai， which can be also used for the screening of followup process and quality control