大鼠急性心肌缺血再灌注.pdfVIP

武警医学 (Medical Journal of the Chinese People ’s Armed Police Forces) Vol. 12 No. 3 2001 - 03 出版 1·31 · 论　　著 大鼠急性心肌缺血再灌注 心肌细胞凋亡与 Bcl - 2/ Bax 蛋白的表达 ① ( ) 武汉科技大学医学院 　周艳玲 　徐 　明 　张玉芹 　张培华 　 武汉 430077 摘 　要 　目的 　为了证实急性心肌缺血再灌注过程中存在着不同程度的心肌细胞凋亡现象 ,初步研究心肌细胞凋 亡与 Bcl - 2/ Bax 蛋白表达的关系。方法 　电镜观察心肌细胞的超微结构变化,抽提心肌组织 DNA 琼脂糖凝胶电泳 , TUNEL 法原位标记凋亡的心肌细胞 ,免疫组化技术和图像分析技术检测心肌细胞内 Bcl - 2/ Bax 蛋白表达。结果 　缺血 及再灌注组电镜观察心肌细胞出现典型凋亡超微结构特征 ,TUNEL 染色可见不同程度的心肌细胞凋亡阳性反应 ,DNA 电 泳显示在缺血再灌注 2 h 组出现明显的 DNA ladder ,再灌注较在缺血组心肌细胞中 Bcl - 2 表达明显降低 ( P 005) ,而 Bax 表达明显增高( P 001) 。结论 　急性心肌缺血及再灌注能诱导不同程度的心肌细胞凋亡 ,Bcl - 2/ Bax 基因对心肌 细胞凋亡的发生有着重要的调控作用。 关键词 　心肌细胞 　凋亡 　缺血/ 再灌注 　大鼠 Apoptosis and expression of Bcl - 2/ Bax proteins in acute ischemic and reperfused rat myocardium Zhou Yanling , Xu Ming , Zhang Yuqin and Zhang Peihua. School of Medicine , Wuhan Umiversity of Science and Technolo gy , Wuhan 430077 , China Abstract 　Objective 　To demonstrate in rats the presence of different degree of myocardiac cell apoptosis during acute ischemia and reperfusion of myocardiam ,and to explore the relations hip between myocardiac cell apoptosis and expression of Bcl - 2/ Bax pro teins. Methods 　The morphologic features of apoptotic myo cardiac cellswere observed by transmission electron microscopy. DNA frag mentations were identified histochemically by TdT - mediated dUTP nickend labeling (UNEL) staining. Expression of Bcl - 2/ Bax pro teins in apoptotic myocardiac cells were observed by immunohistochemical and image analysis. Results 　In both ischemia and reperfusion groups characteristic features of apoptotic myocardiac cells were recognized and apoptotic myo cardiac cells were detected. Reperfusion in creased the OD value of Bax protein ( P 0. 05) ,wh