均匀设计法优化人乳中甲硝唑的检测.PDF

均匀设计法优化人乳中甲硝唑的检测 林红宁1 2 1 2 ，唐铁鑫 ，杨钧 ，刘燕 (1.肇庆市第一人民医院，广东 肇庆 526020 ；2.肇庆医学高等专科学校，广东 肇庆 526020) 摘要：目的 优化人乳中甲硝唑的检测，建立限度检查方法。方法 用均匀设计法确定试验方案，优化从人乳中提取甲 硝唑的条件，用 HPLC 测定甲硝唑。结果 从人乳中提取甲硝唑，不需要加氢氧化钠，也可以不进行超声处理。限量检 测方法的验证试验结果，空白样品色谱图上在与供试品和对照品色谱相应位置处没有色谱峰；检测限0.02 μg·L1 ；在不 同条件下检测同一样品，结果RSD 为 2.2% 。结论 用均匀设计法可以迅速有效地确定检测方法的前处理条件。建立的 人乳中甲硝唑限度检测方法灵敏度高、操作简单、分析速度快、重复性好。 关键词：均匀设计法；人乳；甲硝唑；限度检查；高效液相色谱法 中图分类号：R917.1 文献标志码：B 文章编号：1007-7693(2011)08-0756-03 Optimize the Determination of Metronidazole in Human Milk with Uniform Design Method 1 2 1 2 LIN Hongning , TANG Tiexin , YANG Jun , LIU Yan (1.The First People’s Hospital of Zhaoqing, Zhaoqing 526020, China; 2.Zhaoqing Medical College, Zhaoqing 526020, China) ABSTRACT: OBJECTIVE To optimize the determination of metronidazole in human milk and establish a limit test method. METHODS The experiments scheme was scheduled by uniform design method to improve the extraction of metronidazole from human milk, and the metronidazole was determined by HPLC method. RESULTS For extracting metronidazole from milk, the NaOH should not be added and the super sonic treatment could be removed. In the results of the validation experiments of the limit test method, there was no corresponding peak in the chromatograph of the control comparing with the chromatograph of the sample and the standard, the limit of detection was lower than 0.02 μg·L-1, and the RSD of the test results of the same sample in different conditions was 2.2%. CONCLUSION Uniform design method is effective and rapid to improve the parameters of the test method. The limit test method of metronidazole in human milk established is sensitive, simple, rapid and reproducible. KEY WORDS: unif