RP-HPLC法同时测定复方甘草酸苷片中三组分的含量.PDFVIP

实验研究 RP-HPLC 法同时测定复方甘草酸苷片中三组分的含量 张卫东（北京军区总医院药剂科，北京，100700） 摘要 目的：建立高效液相色谱法同时测定复方甘草酸苷片中甘草酸单铵盐、甘氨酸和蛋氨酸的含量。方法：采用Diamonsil C18 色谱柱（150 mm × 4.6 mm ，5 μm），流动相为乙腈-0.01 mol ·L -1 磷酸溶液（40 ∶ 60），流速1.0 mL ·min -1 ，甘草酸单铵的检测波 长为257 nm，甘氨酸和蛋氨酸的检测波长为262 nm 。结果：甘草酸单铵盐、甘氨酸和蛋氨酸浓度分别在50.52 ~ 505.20 μg · mL -1、10.6 ~ 106.0 μg ·mL -1、10.4 ~ 104.0 μg ·mL -1 范围内呈良好的线性关系；平均回收率分别为（100.32 ± 1.35）%、（101.02 ± 1.80）%和（100.75 ± 1.86）%，RSD 分别为1.35%、1.78%和 1.85% （n = 9）。结论：本法可用于同时测定复方甘草酸苷片中甘草酸 单铵盐、甘氨酸和蛋氨酸的含量，方法简便，结果准确。 关键词 复方甘草酸苷片；甘草酸单铵；甘氨酸；蛋氨酸；柱前衍生化；反相高效液相色谱 中图分类号：R927.2 文献标识码：A 文章编号：1672 - 8157 （2009）03 - 0153 - 03 Determination of Three Kinds of Components in Compound Glycyrrhizin Tablet by RP-HPLC ZHANG Wei-dong(Department of Pharmacy, Beijing Military General Hospital, Beijing, 100700) ABSTRACT Objective: To establish the RP-HPLC method for determination of ammonium glycyrrhizinate, glycine and methion- ine in compound glycyrrhizin tablet. Methods: The column C18 (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.01 mol ·L -1 phosphate solution (40 ∶ 60). The flow rate was 1.0 mL ·min -1, and the detection wavelength were 257 nm and 262 nm respectively. Results: A good linear range of 50.52 ~ 505.20 μg ·mL -1 for ammonium glycyrrhizinate, 10.6 ~ 106.0 μg ·mL -1 for glycine and 10.4 ~ 104.0 μg ·mL -1 for methionine were showed respectively. The recoveries of glycyrrhizin, glycine and methionine were (100.32 ± 1.35)% (RSD = 1.35%), (101.02 ± 1.80)% (RSD = 1.78%) and (100.75 ± 1.86)% (RSD = 1.85%) respec- tively. Conclusion: The method is accurate for determining glycyrrhizin, glycine and methionine in compound glycyrrhizin tablet. KEY WORDS Compound glycyrrhizin tablet; Ammonium glycyrrhizinate; Glycine; Methionine; Precolumn derivatization; RP-HPLC 复方甘草酸苷片是由甘草酸单铵、甘氨酸和蛋氨