两株特境链霉菌产抗MRSA次级代谢产物结构类型的快速鉴定.PDFVIP

·生物化学· 两株特境链霉菌产抗 MRSA 次级代谢产物结构类型的快速鉴定 1 2 1 袁干军 ，李沛波 ，潘微薇 (1.江西农业大学生物科学与工程学院，南昌 330045；2.中山大学生命科学院，广州 510275) 摘要：目的 对链霉菌 ZZ027 和 ZZ021 所产抗耐甲氧西林金黄色葡萄球菌(MRSA)次级代谢产物的结构类型进行快速鉴 定。方法 用 2A 培养基对两链霉菌菌株进行发酵；再采用 HPLC 微分离技术和琼脂扩散法对抗 MRSA 色谱峰进行定位， 然后采用 TLC 对发酵液中抗 MRSA 次级代谢产物进行分离与制备，所得抗 MRSA 组分经薄层显色、IR 和 13C-NMR 谱测 试分析，鉴定其结构类型。结果 链霉菌 ZZ027 和 ZZ021 抗 MRSA 组分的薄层显色、IR 和 13C-NMR 谱特征均与大环内 酯类化合物的相关表征一致。结论 链霉菌 ZZ027 和 ZZ021 所产抗 MRSA 次级代谢产物为大环内酯类化合物。 关键词：链霉菌 ZZ027；链霉菌 ZZ021；大环内酯；结构类型；鉴定 中图分类号：R917.1 文献标志码：B 文章编号：1007-7693(2012)04-0337-05 Quick Identification of Anti-MRSA Secondary Metabolites Produced by Two Streptomyces Strains Isolated from Special Surroundings YUAN Ganjun1, LI Peibo2, PAN Weiwei1(1.College of Bioscience and Engineering, Jiangxi Agricultral University, Nanchang 330045, China; 2.School of Life Science, Sun Yat-sen University, Guangzhou 510275, China) ABSTRACT: OBJECTIVE To quickly identify the structure types of anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) secondary metabolites produced by Streptomyces sp. ZZ027 and Streptomyces sp. ZZ021. METHODS Two strains were fermented with 2A medium, and anti-MRSA chromatographic peaks were localized by HPLC micro-separation technology and agar diffusion method; anti-MRSA ingredients were isolated and prepared by TLC method, and their structure types were deduced by TLC chromogenic reaction, IR and 13C-NMR spectra. RESULTS The characters of chromogenic reaction, IR and 13C-NMR spectra of anti-MRSA ingredients produced by Streptomyces sp. ZZ027 and Streptomyces sp. ZZ021 were consistent with those of macrolides. CONCLUSION Anti-MRSA secondary metabolites produced by Streptomyce