甘草总黄酮氨水提取及超滤纯化工艺研究.PDFVIP

甘草总黄酮氨水提取及超滤纯化工艺研究 ? 1,2 1 1 1,3* 1 1 朱应怀 ，刘晓霞 ，宋晓春 ，魏舒畅 ，金辉 ，王继龙 (1.甘肃中医药大学药学院，兰州 730000；2.甘肃中医药大 学附属医院药学部，兰州 730000；3.甘肃省中医方药挖掘与创新转化重点实验室，兰州 730000) 摘要：目的 建立一种适合于工业化生产的甘草总黄酮提取和纯化方法。方法 以甘草总黄酮的提取率为指标，采用正 交试验确定氨水提取的最佳条件；并以甘草总黄酮的转移率和除杂率为指标，采用正交试验优化最佳超滤工艺参数。结 果 最佳提取条件：0.75%氨水 24 倍，提取 3 次，每次 60 min，总黄酮平均提取率达 83.5%；超滤结果表明：选用 10 nm 的无机陶瓷膜，在操作压力 0.12 MPa 和料液温度 25 ℃条件下甘草总黄酮平均转移率和平均除杂率分别达到 95.6%和 23.3%。结论 该联用技术简便可行，生产成本低，适合工业化生产。 关键词：甘草；总黄酮；提取；超滤；转移率；除杂率 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2017)04-0492-04 DOI: 10.13748/ki.issn1007-7693.2017.04.003 Study on the Extraction of Total Flavonoids from Glycyrrhizae Radix et Rhizoma by Ammonia and the Purification of Ultrafiltration ZHU Yinghuai1,2, LIU Xiaoxia1, SONG Xiaochun1, WEI Shuchang1,3*, JIN Hui1, WANG Jilong1(1.College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China; 2.Department of Pharmacy, The Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China; 3.Key Laboratory of Traditional Chinese Herbs and Prescription Innovation and Transformation of Gansu Province, Lanzhou 730000, China) ABSTRACT: OBJECTIVE To establish a suitable method for industrialized production of total flavonoids extraction and purification from Glycyrrhizae Radix et Rhizoma. METHODS The orthogonal test was used to determine the best extraction conditions within the extraction rate of total flavonoids as the index; and orthogonal experiment was used to optimize the parameters of ultrafiltration process taking the transfer rate of total flavonoids and the ratio of removal of impurity as indexes. RESULTS The best extraction conditions were as follows: 24 times of 0.75% ammonia, extract 3 times, 60 min for each time, the average extraction rate