inactivatingtheAKT-MTORpathway.PDFVIP

BASIC RESEARCH PAPER Autophagy 11:3, 503--515; March 2015; Published with license by Taylor Francis Group, LLC Binding of the pathogen receptor HSP90AA1 to avibirnavirus VP2 induces autophagy by inactivating the AKT-MTOR pathway Boli Hu,1,2,y Yina Zhang,1,y Lu Jia,1 Huansheng Wu,1 Chengfei Fan,1 Yanting Sun,1 Chengjin Ye,1 Min Liao,1 and Jiyong Zhou1,2,* 1Key Laboratory of Animal Virology of Ministry of Agriculture; Zhejiang University; Hangzhou, China; 2College of Veterinary Medicine; Nanjing Agricultural University; Nanjing, China yThese authors contributed equally to this work. Keywords: AKT-MTOR pathway, autophagy, avibirnavirus, HSP90AA1, viral protein VP2 Abbreviations: ANOVA, analysis of variance; ATG5, autophagy-related 5; BCA, bicinchoninic acid; BECN1, Beclin 1, autophagy- related; cDNA, complementary DNA; CoIP, coimmunoprecipitation; DMEM, Dulbecco’s modi?ed Eagle’s medium; dsRNA, dou- ble-stranded RNA; EBSS, Earle’s balanced salt solution; EIF2AK2, eukaryotic translation initiation factor 2-alpha kinase 2; EIF2S1, eukaryotic translation initiation factor 2, subunit 1 alpha; eGFP, enhanced green ?uorescent protein; ER, endoplasmic reticulum; Gg, Gallus gallus (chicken); GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GOPC, golgi-associated PDZ and coiled-coil motif containing; GST, glutathione S-transferase; HE-IBDV, heat-inactivated IBDV; hpi, hours post-infection; Hs, Homo sapiens (human); HSP90AA1, heat shock protein 90 kDa alpha (cytosolic), class A member 1; HSV-1, herpes simplex virus 1; IBDV, infec- tious bursal disease virus; IgG, immunoglobulin G; LPS, lipopolysaccharide; mAb, monoclonal antibody; MAP1LC3/LC3, microtu- bule-associated protein 1 light chain 3; MO