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Characterization and Expression Analysis Of Six Chitinase From Microdera Punctipennis Low Tempe
438
CryoLetters 35 (5), 438-448 (2014)
? CryoLetters, businessoffice@
CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE
GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE
TO LOW TEMPERATURE
Xueying Lu
1,2
, Jieqiong Li
1
, Xiaoning Liu
1
, Xiaobo Li
2
and Ji Ma
1
1
Key Laboratory of Xinjiang Biological Resources and Genetic Engineering,
College of Life Science and Technology, Xinjiang University, Urumqi, China.
2
Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical
Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.
*Corresponding author email: majiuci@
Abstract
BACKGROUND: Chitinase is responsible for chitin metabolism in a wide range of organisms.
However, current knowledge on insect chitinase and their possible functions in relation to low
temperature stress is very limited. OBJECTIVE: Six chitinase genes from cold treated desert beetle
Microdera punctipennis obtained by RNA-seq technology were characterized, and their expression
patterns in different tissues and in response to cold were investigated. METHODS: Multiple sequence
alignment was carried out using ClustalW1.81 and Phylogenetic trees were generated by MEGA5. The
expression patterns were studied by quantitative real-time PCR. RESULTS: These genes were belong
to three different chitinase groups. Almost all of them were highly expressed in midgut, and some are
expressed in fat body or hindgut. Subzero-4oC had stronger effect than 4oC in inducing chitinase
expression. CONCLUSION: The tissue specific and cold inducible expressions suggest that the
chitinases may have diverse functions and play roles in insect cold adaptation.
Keywords: Microdera punctipennis, chitinase genes, cold stress, tissue-specific gene expression.
INTRODUCTION
Chitinases are glycosyl hydrolases, which
hydrolyze β-1,4-glycosidic bonds of chitin.
Diverse organisms, such as arthropods, bacteria,
fungi and plants,
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