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Effect of chitosan-alginate nanoparticles and ultrasound on the efficiency of gene.pdf

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Effect of chitosan-alginate nanoparticles and ultrasound on the efficiency of gene

THE JOURNAL OF GENE MEDICINE R E S E A R C H A R T I C L E J Gene Med 2010; 12: 168–179. Published online 26 November 2009 in Wiley InterScience () DOI: 10.1002/jgm.1418 Effect of chitosan-alginate nanoparticles and ultrasound on the efficiency of gene transfection of human cancer cells Shu-Jyuan Yang1? Szu-Min Chang1? Kun-Che Tsai1 Wen-Shiang Chen2 Feng-Huei Lin1 Ming-Jium Shieh1,3* 1Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan 2Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan 3Department of Oncology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan *Correspondence to: Ming-Jium Shieh, Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan. E-mail: soloman@.tw; image 0120@.tw ?Both investigators contributed equally and should be considered as senior authors. Received: 8 April 2009 Revised: 18 September 2009 Accepted: 23 October 2009 Abstract Background Gene therapy has been used to treat a variety of health problems, but transfection inefficiency and the lack of safe vectors have limited clinical progress. Fabrication of a vector that is safe and has high transfection efficiency is crucial for the development of successful gene therapy. The present study aimed to synthesize chitosan-alginate nanoparticles that can be used as carriers of the pAcGFP1-C1 plasmid and to use these nanoparticles with an ultrasound protocol to achieve high efficiency gene transfection. Methods Chitosan was complexed with alginate and the pAcGFP1- C1 plasmid at different charge ratios to create chitosan-alginate-DNA nanoparticles (CADNs). The average particle size and loading efficiency were measured. Plasmid DNA retardation and integrity were analysed on 1% agarose gels. The effect of CADNs and ultrasound on the efficiency of t