高效液相色谱法测定人血浆中左氧氟沙星浓度.PDFVIP

临床药学 高效液相色谱法测定人血浆中左氧氟沙星浓度 邓 鸣, 刘建芳, 于 洋, 侯艳宁 (白求恩国际和平医院临床药理室, 石家庄 050082) 摘要: 建立测定人血浆中左氧氟沙星浓度的高效液相色谱法, 并应用于人体药代动力学研究。血浆样品 中加入内标洛美沙星后用乙腈沉淀蛋白, 上清液用氮气吹干。色谱柱为 Diamonsil C18柱, 流动相为 0 01 mol L - 1 磷酸二氢钾缓冲液 (含三乙胺 0 3% , 磷酸调至 pH 3 20) –乙腈 ( 83 17) , 流速为 1 0m l min - 1 。紫外检测波长 294 nm。血浆中内源性物质对样品测定无干扰。本方法线性范围为 0 05~ 5 g ml- 1 ( r= 0 9991) , 最低定量浓度为 0 05 g ml- 1 , 提取回收率大于 85%, 方法回收率为 99 7% ~ 103 3%, 日内、日间 RSD 均小于 4%。本法简便、准确, 适用于左氧氟沙星药代动力学的研究。 关键词: 左氧氟沙星; 高效液相色谱法; 药代动力学 中图分类号: R969 1 文献标识码: A 文章编号: 1002- 7777 ( 2006) 07- 0439- 04 Determination of Levofloxacin in Human Plasma by HPLC Deng M ing , L iu Jianfang , Yu Yang and Hou Yanning ( Department of Clinical Pharmacolo gy, Bethune Internat ional Peace Hospital, Hebei Pr ovince, Shijiazhuang , 050082) ABSTRACT A HPLC method w as established for the determ inat ion of levof lox acin in human plasma and fo r human pharmacokinetics study . T he internal standard lomeflox acin w as added in the plasma sample, and protein w as precipitated w ith acetonit rile. The supernatant w as evaporated by nit rogen st ream . A Diamonsil C18 column w as used w ith 0 01 mol L- 1 potassium dihydrogen pho sphate solut ion ( contained 0 3% triethy lam ine, pH was adjusted to 3 20 w ith phosphoric acid) and acetonit rile ( 83 17) as mobile phase. T he f low rate w as 1 0 ml min- 1 and the U V detect ion w aveleng th was 294 nm. T he endogenous substance in plasma did not interfere with the determination o f the samples. T he linear range w as 0 05~ 5 g ml - 1 ( r= 0. 9991) , and the limit of quant itat ion w as 0 05 g m l - 1 . T he ex t ract ion recovery w as more than 85% and the method recovery w as 99 7~ 103 3%. Both w ithin- day RSD and betw een- day RSD wer e less than 4% . T he method w as simple and accurate. It w as suitable for the pharmacokinetic study of levof lox acin. KEYWORDS levo flo xacin; HPLC; pharmacokinet ic 左氧氟沙星 ( levof lox acin) 为日本第一制药 株式会社开发的第三代喹诺酮类药物, 为氧氟沙星 的左旋体, 其体外抗菌活性约为氧氟沙星的两倍, 左氧氟沙星具有抗菌谱广, 抗菌作用强, 而毒副作 用小的特点, 对大多数肠杆菌科