YX2014016.docVIP

瘦素基因敲除肥胖大鼠的血糖及病理长期观察 张丽 关菲菲 张旭 陈炜 孙彩显 张连峰 (中国医学科学院，北京协和医学院，医学实验动物研究所，卫生部人类疾病比较医学重点实验室，北京100021) 【摘要】目的：Leptin基因敲除SD大鼠方法：eptin+/+大鼠和Leptin-/-大鼠肝脏中Leptin的表达。利用称量方法测定Leptin基因敲除的SD大鼠Leptin-/-）1,3,6,8月龄的体重变化，Leptin-/-大鼠Leptin-/-大鼠胰腺及肝脏的病理学变化。结果：Leptin-/-大鼠从开始出现显著的体重增加，。 Leptin-/-雌鼠结论: Leptin-/-大鼠基金项目国家“重大新药创制”科技重大专项课题“啮齿类研发平台创新药物研究开发术平台建设”(2011ZX09307-302); 国家科技支撑计划课题“神经和代谢疾病基因工程模型的建立”(2012BAI39B02)作者简介女，博士，E-mail:通作者张连峰，Tel: 86-010E-mail: Zhanglf@cnilas.orgI Long-time observation of blood glucose and pathological phenotype of Leptin knockout obese rats ZHANG Li,GUAN Fei-fei,ZHANG Xu,CHEN Wei,SUN Cai-xian,ZHANG Lian-feng (Key Laboratory of Human Diseases Comparative Medicine，Ministry of Health；Institute of Laboratory Animal Science，Chinese Academy of Medical Sciences(CAMS)＆Comparative Medicine Centre，Peking Union Medical Coege(PUMC)，Beijing 100021，China)Abstract Objective: The long-term observation of fasting blood glucose and pathological phenotypes of Leptin knockout SD rats were performed to obtain more physiological data available for the users of this animal model. Methods: The protein expression levels in liver tissues were determined by western blot. Body weight of Leptin knockout rats (Leptin-/-) and littermate lean rats (Leptin+/+) were weighed up at 1,3,6,8 months of age. Fasting blood glucose of Leptin+/+ rats and Leptin-/- rats at 1,3,6,8 months of age were measured using OneTouch? brand?blood?glucose?monitoring?systems. Pathological changes of pancreas and livers of Leptin-/- rats were observed by the method of HE staining and Immunohistochemistry (IHC). Results: Short null Lepin proteins were expressed in liver tissues from Leptin-/- rats. Leptin-/- rats become heavier than Leptin+/+ rats since they were one month old. The body weight of Leptin-/- rats at 8 months of age was twice as heavy as Leptin+/+ rats, female Leptin-/- rats weighing 884g, and male Leptin-/- rats weighing 1200g. Overt hyperglycemia was observed during the first month af