Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxic–biological contact oxidation system.pdfVIP
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Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxic–biological contact oxidation system.pdf
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Journal of Bioscience and Bioengineering VOL. 122 No. 4, 456e466, 2016
Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxicebiological contact oxidation
system
Miao Zhang, Qing Yang, Jianhua Zhang, Cong Wang, Shuying Wang, and Yongzhen Peng*
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China
Received 21 December 2015; accepted 22 March 2016
Available online 25 April 2016
A two-sludge system consisting of anaerobic anoxic oxicebiological contact oxidation (A2/OeBCO) was developed to
treat domestic wastewater with a low carbon/nitrogen (COD/TN) ratio (around 3.21) by shortening sludge retention time (SRT) for phosphorus accumulating organisms (PAOs) in the A2/O reactor and prolonging SRT for nitri?ers in the BCO
reactor. Speci?cally, the BCO reactor was composed of three stages in series (N1, N2 and N3), so that simultaneous nitrogen and phosphorus removals by denitrifying PAOs (DNPAOs) was achieved in the A2/O reactor with NOxLeN as the
electron acceptor from the BCO reactor. Long term operational tests (600 days) were conducted with various operational
parameters [e.g., hydraulic retention time (HRTs), nitrate recycling ratio (Rs), volume ratio (Vs)] to examine the deni-
trifying phosphorus 8 h, Rs of 300% and
removal performance. The system exhibited the highest removal Vs of 2:4:1. The optimal TN and PO43LeP removals were 80.30%
of TN and PO4 and 96.61% at
3LeP at the low COD/TN
HRTs of of 3.21.
The species diversity and microbial community examined by the Illumina MiSeq method demonstrated the fact of two-
sludge system, and the improved community structure by long-term optimization was prominent comparing with the seed sludge. Additionally, Accumulibacter and Dechloromonas were the dominant functional PAOs with 25.74% in the A2/
O reactor
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