优秀毕业论文（设计）_生菜中多酚的提取及含量的测定.docxVIP

生菜中多酚的提取及含量的测定摘要本论文主要是围绕生菜叶中的多酚的提取及含量的测定展开研究。植物多酚作为一种天然大分子化合物广泛存在于植物体内，含量仅次于木质素、纤维素和半纤维素，并在石油开采、化妆品添加剂、金属防腐、食品添加剂、木材加工等领域得到很好地应用。在崇尚环保，提倡绿色的今天，植物多酚得的研究取得了很好地研究。在多酚的提取时使用了有机溶剂提取的方法。有机溶剂提取时使用95%乙醇，其最佳测定条件是生菜在95%乙醇90ml和10ml蒸馏水中，室温下密封浸泡24小时。在多酚的测定条件时使用酒石酸亚铁分光光度法，其测定条件是：溶液pH=7.5，显色剂5ml，在此条件下，测得生菜多酚的含量为6.0mg/L。关键词：生菜 植物多酚 多酚提取 含量测定Extraction and content determination of polyphenols in lettuceAbstractThis paper is mainly on the extraction and determination of polyphenols in lettuce research.Plant polyphenols as a natural high molecular compound, which widely exists in plants and content after lignin, cellulose and hemicellulose, and in oil exploitation, cosmetic additives, metal anti-corrosion, food additives, wood processing field obtained very good application. In respect of environmental protection, the promotion of green today, the study of plant polyphenols has made a good study.Organic solvent extraction method was used in the extraction of polyphenols. Organic solvent extraction using 95% ethanol, the optimal conditions for the determination is lettuce in 95% ethanol 90ml and 10 ml of distilled water, room temperature sealed and soaked for 24 hours. Polyphenol determination conditions when using ferrous tartrate spectrophotometry. The determination conditions are: solution pH = 7.5, chromogenic reagent 5ml, under these conditions, the measured lettuce polyphenol content was 6.0 mg / L.Keywords:Lettuce Plant polyphenol Purification of polyphenols Determination of content目录一、绪论11.1生菜的营养价值及植物多酚组成与结构11.2植物多酚的组成与结构11.3植物多酚的化学性质21.4植物多酚的提取[24]21.5 植物多酚含量的测定31.6 pH磷酸缓冲液的配置41.7本论文的研究目的5二、生菜中多酚的提取及含量的测定62.1生菜中多酚的提取62.2分光光度法测定生菜提取液中植物多酚的含量62.2.1酒石酸亚铁分光光度法测定提取液中植物多酚的含量62.2.2样品试液的制备72.2.3实验方法82.2.4 有机溶剂提取82.2.5植物多酚的含量测定条件讨论102.2.5.1磷酸缓冲液pH值得影响102.2.5.2显色剂用量的影响112.2.5.3标准曲线的绘制122.2.5.4样品多酚总量的测定方法12三、结论14致谢15参考文献16一、绪论1.1生菜的营养价值及植物多酚组成与结构生菜，又名莴苣，是叶用莴苣的俗称，属于菊科莴苣属。因为菜茎叶中含有莴苣素，故生菜吃起来味稍苦。生菜具有镇痛催眠，降低胆固醇，治疗效果和神经衰弱。生菜甘露醇等有效点具有利尿，促进血液循环。生菜中含有一种叫的“干扰素诱生剂”能够刺激人体正常细胞从而产生干扰素[1]。功效