海洋蛭弧菌的分离鉴定及其对副溶血弧菌的作用.docVIP

海洋蛭弧菌的分离鉴定及其对副溶血弧菌的作用储卫华朱卫中国药科大学生命科学与技术学院南京210009) (2. 江苏星海动物药业科技有限公司 江苏 东台 224233) 摘 要: 蛭弧菌广泛存在于自然水体, 具有噬菌的特性, 对水体中细菌数量控制具调节作用。以副溶血弧菌为宿主菌利用双层琼脂法, 从海洋水体中分离出15株具有噬菌作用的细菌, 对形成噬菌斑能力最强的菌株进行16S rDNA扩增, 确认为蛭弧菌Bd-M1。Bd-M1对大多数海水养殖动物病原菌有裂解作用, 裂解率在%(20/22)以上, 模拟水环境实验发现, 蛭弧菌对副溶血弧菌有较强的裂解和净化作用, 102 h内能使副溶血弧菌从3.0(108/mL下降到8.7×103/mL。动物实验表明蛭弧菌能有效预防对虾弧菌病的发生表明蛭弧菌有望成为水产动物疾病防治的一种有效的生物制剂。副溶血弧菌, 蛭弧菌, 对虾, 生物防治Isolation, Identification of Marine Bdellovibrios and Its Effect on Vibrio parahaemolyticus CHU Wei-Hua1* ZHU Wei1 KANG Chun-Tao2 (1. Department of Microbiology, School of Life Science Technology, China Pharmaceutical University,Nanjing, Jiangsu 210009, China) (2. Jiangsu Xinghai Veterinary Pharmaceutical Co., Ltd., Dongtai, Jiangsu 224233, China) Abstract: Bdellovibrios are obligate predatory bacteria, widely distribute in nature and regulate the counts of bacterium in water. Fifteen strains of predatory bacteria were isolated from marine environment using Vibrio parahaemolyticus DX-1 as host and one of them formed large plaques designated as Bd-M1. The strain was confirmed by Bdellovibrio species-specific 16S rDNA fragment amplified using PCR method. Determination of host range on species of fish pathogenic bacteria (Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio anguillarum, Vibrio vulnificus and Edwardsiella tarta) indicated that Bd-M1 was able to parasitize 20 strains out of a total of 22 tested. Co-culture experiment shown that Bd-M1 have purification function on Vibrio parahaemolyticus, the number of V. parahaemolyticus decreased from 3.0( 108 CFU/mL to 8.7×103 CFU/mL during 102 h. Immersion Penaeus vannamei in water at different concentration of Bd-M1 was used in protection against experimental infection with V. parahaemolyticus DX-1 infection. Results shown that the mortality of groups bathed with Bd-M1 was lower than that group without Bd-M1. These results suggest that it ma