卵巢癌CD90+肿瘤干细胞的生物学特性.docVIP

卵巢癌CD90+肿瘤干细胞的生物学特性 刘智慧1，向志辉2(1武汉市妇女儿童医疗保健中心，湖北省武汉市 430016；2华中科技大学同济医学院附属武汉市中心医院，湖北省武汉市 430000) 引用本文：刘智慧，向志辉. 卵巢癌CD90+肿瘤干细胞的生物学特性[J].中国组织工程研究，2016，20(10):1468-1473. DOI: 10.3969/j.issn.2095-4344.2016.10.014 ORCID: 0000-0003-1998-4794 (刘智慧) 文章快速阅读： 文题释义： CD90蛋白Biological features of CD90+ tumor stem cells in ovarian cancer Liu Zhi-hui1, Xiang Zhi-hui2 (1Wuhan Women and Children Medical Care Center, Wuhan 430016, Hubei Province, China; 2the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science Technology, Wuhan 430000, Hubei Province, China) Abstract BACKGROUND: Thinking from ovarian cancer stem cell theory shows that: in the tumor cells, there are a fraction of stem cells with self-renewing ability and multipotent differentiation, which are the root causes of ovarian cancer recurrence and drug resistance. Studies have shown that CD90 can be used as a surface marker of mesenchymal stem cells and stem cells of other cancers. OBJECTIVE: To explore the biological features of CD90+ tumor cells from ovarian cancer tissues. METHODS: Primary ovarian cancer cells were isolated from the abdominal dropsy of ovarian cancer patients to sort CD90+ and CD90- cells using flow cytometry. RT-PCR was used to detect expressions of stem cell-related genes and epithelial to mesenchymal transition-related genes. Cell invasion was observed by Transwell invasion assay, cell proliferation and differentiation observed by clone formation assay, stem cell potential observed by suspension sphere-forming assay, and tumor formation rate observed by in vivo tumorigenicity experiment. RESULTS AND CONCLUSION: Compared with the CD90- cells, the expressions of CD44, CD133, ALDH1, N-cad and Vimentine were significantly higher in the CD90+ cells (P 0.05), but the expression of E-cad was significantly decreased in the CD90+ cells (P 0.05). Tumor formation rates of CD90- and CD90+ cells were increased significantly with the increase of seeded