《气相色谱法测定大鼠粪便短链脂肪酸色谱条件的优化毕业论文》.docxVIP

编号：100毕业论文（设计）题目气相色谱法测定大鼠粪便短链脂肪酸色谱条件的优化院（系） ：食品学院 专业 ：食品科学与工程学生姓名 ：玛依努尔·买买提成绩：指导教师肖俊松（职称）副教授2014年05月诚信声明本人郑重声明：所呈交的毕业设计（论文）是我个人在导师指导下,由我本人独立完成。有关观点、方法、数据和文献等的引用已在文中指出,并与参考文献相对应。我承诺，论文中的所有内容均真实、可信。如在文中涉及到抄袭或剽窃行为，本人愿承担由此而造成的一切后果及责任。毕业论文（设计）作者签名：签名日期： 年 月 日摘要气相色谱法是测定短链脂肪酸的常用方法。为了研究原花青素对肥胖大鼠肠道菌群的影响，需要对大鼠粪便中的短链脂肪酸定性和定量。本论文对影响短链脂肪酸测定的两个关键因素：样品前处理方法以及色谱条件进行了优化，比较了三种色谱条件以及四种样品前处理方法：盐酸水提法、偏磷酸处理法、水提乙醚萃取法和固相微萃取法。结果表明最优样品前处理法为盐酸水提乙醚萃取法，优化的色谱条件为：色谱条件B。色谱条件B：柱型号JM122-7032；柱规格为30.0m×250mm×0.25μm的DB-WAX毛细管柱。测定采用程序升温，初温100℃，保持0.5min；以8℃/min速度升至180℃，保持1min；然后以20℃/min速度升至200℃，保持5min，分流比为10:1，载气为He，流速为1.2mL/min，FID检测器温度为250℃，进样口温度为250℃。测定了15个大鼠粪便样品中短链脂肪酸的含量。关键词：短链脂肪酸；大鼠粪便；气相色谱；样品前处理ABSTRACTGas chromatography is a common method for the determination of short-chain fatty acids.To study the effect of proanthocyanidins on gut microbiotaof rats,the short-chain fatty acids were qualified and quantified.Two key factors influencing the determination of short-chain fatty acids,i.e.,sample preparation methods and chromatographic conditions,were optimized,and totally3 chromatographic conditions and 4 sample preparation methods were compared,i.e.,hydrochloric acid-acidified water extraction,metaphosphoric acidextraction, acidified water extraction followed by ether extraction and solid phase micro-extraction.Results showed that optimum preparation method was hydrochloric acid-acidified water extraction,and the optimum GC conditions were as follows:Column Type JM122-7032;30.0m×250mm×0.25μm the DB-WAX capillary column.Determined by temperature-programmed,initial temperature 100℃,keeping 0.5min;with 8℃/min rate rose to 180℃,keeping 1min;then with 20℃/min rate rose to 200℃,keeping 5min,split ratio of 10:1, The carrier gas is He,flow rate 1.2mL/min,FID detector temperature is 250℃,inlet temperature is 250℃.Totally 15 rats feces samples were determined.Key word:Short-chain fatty acids;rat feces;Gas chromatography; Sample preparation method目录第一章 绪论21.1 短链脂肪酸与其生理功能21.2 短链脂肪酸的生成与代谢21.3 短链脂肪酸测定方法21.3.1 前处理