人源噬菌体抗体库的构建及抗beta-淀粉样蛋白寡聚体单链抗体的筛选.docVIP

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers Zuanning Yuan1, 2, Minge Du1, 2, Yiwen Chen3, Fei Dou1, 2 1 Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China2 Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China3 Geriatric Internal Department of Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China 窗体顶端 Abstract: Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli-gomers and constructed a na?ve human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe-cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked immunosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 te-tramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc-cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers. Key words： neural regeneration; amyloid-beta; Alzheimer’s disease; oligomer; single-domain antibody; phage display; antibody library construction; alpha-synuclein; Parkinson’s disease; humanized antibody; immunotherapy; grants-supported paper; neuroregeneration; 窗体底端 Acceptance date: 2013-04-25 Cite this article: Zuanning Yuan, Minge Du, Yiwen Chen, Fei Dou. Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers[J]. Neural Regeneration Research, 2013, 8 33 : 3107-3115.