20120812ISQonsitebasictraining要点.pptVIP

首先定义内标，重量和单位 其次逐个设定每个目标成分，并且选择此成分的内标； 权重：一般选择1/X，标准曲线倾向于低浓度的点，可以根据具体情况调整； Origin：Ignore 忽略原点 Response：Area 根据峰面积的响应 浓度水平Level设定；质控样品QC设定;可以右键点击Copy Levels to All Target Compounds，然后根据具体情况再对每个目标成分的水平进行修改 首先打开或新建一个序列文件。 样品还未分析，序列中选择Instrument Method和Process Method，点击运行样品，再选中Quan，样品分析完即被处理。 样品已经分析完，打开需要处理的序列文件，点击Batch Reprocess，检查 Quan的Check Box是否选中，这时只处理数据，不采集数据。 Must save sequence to open in quan browser, or can open individual quan result files. 右键点击上图表格，可以对表格内容重新设定 进入User Peak Detection Settings可以重新更改积分参数 点击Show Calibration Curve 质谱图可以切换为校正曲线 Select the mass range you would like to monitor in the scan parameters and add segment which you would like to acquire data for this mass range. A different multiplier can be set for each segment. Scan time dictates how long to take over one full scan of the mass range selected. To optimise sensitivity and quantitative accuracy/precision, use Segment and monitor only a select number of ions (Scan Parameters) in a specific time window. Further enhancement in sensitivity/specificity can be achieved by adjusting the dwell time and span width used to monitor an ion. Note each Segment can have a unique Multiplier Voltage. By selecting Add Event we can add Scan 1 for SIM and Scan 2 for Full Scan to an experiment. Alternate data points would then arise from each experiment. JUST BE WARY – by adding more scan events, we reduce the number of data points per experiment per second! 在采集序列编辑器中需要完全填写的是：样品类型，文件名，路径，仪器方法，样品位置和进样体积。 注意：样品输入目录中可以有不同的路径﹙记录相应的原始数据﹚和不同的仪器方法。 对于批处理或者处理序列来说，需要输入处理方法和样品浓度水平。 注意：与数据采集序列不同，当以批处理来处理样品序列，所有的样品输入目录中必须是同一个处理方法。 Required entries in the acquisition sequence editor are: Sample Type, File Name, Path, Instrument Method, Position, and Injection Volume. Note: Sample entries can be associated with different paths (for recording corresponding raw data) and different instrument methods. Required entries for a Batch Reprocessing/Processing Sequence are: Processing Method and Level. Note: As opposed to