《bti730 4330..4337-Vol. 21 no. 24 2005, pages 4330ndash;43》.pdfVIP

Vol. 21 no. 24 2005, pages 4330–4337 BIOINFORMATICS ORIGINAL PAPER doi:10.1093/bioinformatics/bti730 Sequence analysis Integrated minimum-set primers and unique probe design algorithms for differential detection on symptom-related pathogens 1, 3, 1 1,2 Yu-Cheng Huang †, Chun-Fan Chang †, Chen-hsiung Chan , Tze-Jung Yeh , Ya-Chun Chang2 , Chaur-Chin Chen4 and Cheng-Yan Kao1,5, 1Bioinformatics Laboratory, Department of Computer Science and Information Engineering and 2Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan, 3Breed-Use-Special Laboratory, Center of Agriculture Hierarchical Utilization, Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan, 4Department of Computer Science, National Tsing-Hua University, Hsinchu, Taiwan and 5Institute for Information Industry, Taipei, Taiwan Received on May 27, 2005; revised on October 14, 2005; accepted on October 18, 2005 Advance Access publication October 25, 2005 ABSTRACT MMA-PAH system have successfully validated the specificity and Motivation: Differential detection on symptom-related pathogens sensitivity of the primers/probes designed with our integrated (SRP) is critical for fast identification and accurate control against PDA-MS/UniQ method. epidemic diseases. Conventional polymerase chain reaction (PCR) Contact: cykao@.tw requires a large number of unique primers to amplify selected SRP Supplementary i