浅刺调督综合疗法对脑梗塞大鼠神经细胞保护作用的实验的研究.pdf

目 录 中文摘要····················································1 英文摘要 ····················································2 引 言 ·····················································4 正 文 ·····················································5 1.实验材料和方法············································5 1.1 实验材料·················································5 1.1.1 实验动物···············································5 1.1.2 药物与试剂·············································5 1.1.3 主要仪器 ···············································5 1.2 实验方法·················································5 1.2.1 脑梗塞动物模型制作法 ···································5 1.2.2模型判断标准 ···········································6 1.2.3 动物分组 ···············································6 1.2.4 操作方法 ···············································6 1.3 取材与观察指标 ···········································7 1.3.1 脑组织的采集和固定 ·····································7 1.3.2脑组织BDNF的检测方法 ···································8 1.3.3脑组织c-Fos的检测方法 ··································8 1.3.4观察指标 ···············································8 1.4 统计学方法 ···············································9 2.实验结果··················································9 2.1 实验动物数量分析·········································9 2.2 大鼠神经功能评定·········································9 2.3 苏木素-伊红染色(HE-染色) ·······························10 2.4三个时间点各组大鼠脑组织BDNF表达比较····················10 2.5三个时间点各组大鼠脑组织c-Fos表达比较···················11 3.讨 论 ···················································12 3.1祖国医学对脑梗塞的认识 ··································12 3.2现代医学对脑梗塞的认识 ··································15 3.3 关于脑梗塞模型的制作和神经功能的评价 ····················18 3.4 浅刺调督综合疗法的理论及依据 ····························18 3.4.1 浅刺针法的理论和依据 ··································18 3.4.2调督法治脑梗塞的理论及依据 ····························20 3.4.3 辅以补阳还伍汤的理论和依据 ····························21 3.5脑梗塞与脑源性神经生长因子BDNF 的关系···········