Potential Significance of EMP3 in Patients with Upper Urinary Tract Urothelial Carcinoma Crosstalk with ErbB2-PI3K-Akt Pathway》.pdfVIP

Investigative Urology Potential Significance of EMP3 in Patients with Upper Urinary Tract Urothelial Carcinoma: Crosstalk with ErbB2-PI3K-Akt Pathway Yi-Wen Wang, Wei-Ming Li, Wen-Jeng Wu, Chee-Yin Chai, Hsiao-Sheng Liu, Ming-Derg Lai and Nan-Haw Chow* From the Institute of Basic Medical Sciences (YWW, HSL, MDL, NHC), Department of Microbiology and Immunology (HSL) and Departments of Biochemistry and Molecular Biology (MDL) and Pathology (NHC), College of Medicine, National Cheng Kung University, Tainan and Departments of Urology (WML, WJW) and Pathology (CYC), Kaohsiung Medical University Hospital, Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital (WJW) and Department of Urology, Faculty of Medicine, College of Medicine (WJW), Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China Purpose: Upper urinary tract (pyelocalyceal cavities and ureter) urothelial Abbreviations carcinoma is a relatively rare neoplastic disease. Although diagnosis and treat- and Acronyms ment of this tumor variant have improved significantly, accurate risk stratifi- EMP3 ¼ epithelial membrane cation remains a challenge. To identify the putative oncogene involved in protein 3 urothelial carcinoma progression we performed bioinformatics guided experi- FAK ¼ focal adhesion kinase mental investigation targeting chromosome 19q13. PCR ¼ polymerase chain reaction Materials and Methods: We investigated the effects of EMP3 on cancer cell ROCK ¼ Rho-associated growth, migration and adhesion in transfection and siRNA experiments coiled-coil containing protein in vitro. Crosstalk