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PD150606 protects against ischemiareperfusion injury by preventing μ-calpain-induced mitochondrial apoptosis》.pdf
Archives of Biochemistry and Biophysics 586 (2015) 1–9 Contents lists available at ScienceDirect Archives of Biochemistry and Biophysics journal homepage: /locate/yabbi PD150606 protects against ischemia/reperfusion injury by preventing l-calpain-induced mitochondrial apoptosis Tao Luo a,d,1, Rongchuan Yue a,1, Houxiang Hu a,b,⇑, Zhou Zhou c, Kai Hang Yiu e, Shuang Zhang a, Lei Xu a, Ke Li a, Zhengping Yu c a Department of Cardiology, North Sichuan Medical College First Affiliated Hospital, Nanchong 637000, Sichuan, China b Center for Medical Research, North Sichuan Medical College First Affiliated Hospital, Nanchong 637000, Sichuan, China c Department of Occupational Health, Third Military Medical University, Chongqing 400038, China d Department of Cardiology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China e Department of Medicine, University of Hong Kong, Hong Kong 999077, China a r t i c l e i n f o a b s t r a c t Article history: Calpain plays an important role in myocardial ischemia/reperfusion (I/R) injury. PD150606, a nonpeptide, Received 11 April 2015 cell-permeable and noncompetitive calpain inhibitor, has been shown to have protective properties in and in revised form 15 May 2015 ischemic disease. The aims of the present study were to investigate whether PD150606 could alleviate Available online 16 June 2015 myocardial I/R injury and to examine the possible mechanisms involved. The I/R model was established in vivo in C57BL/6 mice and in vitro using neonatal mouse cardiomyocytes, respectively. To evaluate the
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