Peroxisome proliferator-activated receptor γ1 and γ2 isoforms alter lipogenic gene networks in goat mammary epithelial cells to different extents》.pdfVIP

J. Dairy Sci. 97 :5437–5447 /10.3168/jds.2013-7863 © American Dairy Science Association® , 2014. gene networks in goat mammary epithelial cells to different extents H. B. Shi,* W. S. Zhao,*† J. Luo ,*1 D. W. Yao,* Y. T. Sun,* J. Li,* H. P. Shi,* and J. J. Loor†1 *Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest AF University, Yangling, Shaanxi, 712100, China †Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801 ABSTRACT Key words: fatty acid metabolism , mammary gland, lactation , gene network , nuclear receptor In nonruminants, the alternative splicing of peroxi- some proliferator-activated receptor γ (PPARG) gener- INTRODUCTION ates PPARG1 and PPARG2 isoforms. Although tran- scriptional control differences between isoforms have Peroxisome proliferator-activated receptor gamma been reported in human adipose tissue, their roles in (PPARG) is a ligand-dependent member of the nu- ruminant mammary cells are not well known. To assess clear hormone receptor superfamily with a key role in which of these isoforms is more closely associated with fat cell differentiation and lipid filling (Rosen and Spie- the regulation of mammary lipogenic pathways, their gelman, 2001). Various long-chain fatty acids (LCFA; tissue distribution was analyzed and the expression Kadegowda et al., 2009) and chemical compounds (e.g., of key genes regulating lipogenic gene networks was thiazolidinediones; Khandoudi et al., 2002) can bind measured after overexpression of the 2 isoforms in goat t