Inhibition on hepatitis B virus e-gene expression of 10–23 DNAzyme delivered by novel chitosan oligosaccharide–stearic acid micelles》.pdfVIP

Carbohydrate Polymers 87 (2012) 1342–1347 Contents lists available at SciVerse ScienceDirect Carbohydrate Polymers j o u r na l h o me p a g e : w w w. elsev /locate/car bpol Inhibition on hepatitis B virus e-gene expression of 10–23 DNAzyme delivered by novel chitosan oligosaccharide–stearic acid micelles Jing Miao a , Xing-guo Zhang a,∗ , Yun Hong a , Yue-feng Rao a , Qian Li a , Xian-ji Xie a , Jian-er Wo a,b , Min-wei Li a,b a Department of Pharmacy, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, PR China b State Key Lab for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, PR China a r t i c l e i n f o a b s t r a c t Article history: 10–23 DNAzyme (DrzBC) could block the expression of HBV e-gene with application limit of depen- Received 15 July 2011 dence on exogenous delivery. Stearic acid grafted chitosan oligosaccharide (CSSA) could self-aggregate Received in revised form 31 August 2011 to form micelles in aqueous medium and bind with DrzBC by electrostatic interaction. Compared with Accepted 7 September 2011 TM Lipofectamine 2000, the CSSA micelles showed much lower cytotoxicity (412.5 g/mL) and advan- Available online 14 September 2011 TM taged target in subcellular organelles-cytoplasm. Both including DrzBC of 1 mol/L, Lipofectamine