Identification of conserved and novel microRNAs in Manduca sexta and their possible roles in the expression regulation of immunity-related genes》.pdfVIP

Insect Biochemistry and Molecular Biology 47 (2014) 12e22 Contents lists available at ScienceDirect Insect Biochemistry and Molecular Biology journal homepage: www.els /locate/ibmb Identification of conserved and novel microRNAs in Manduca sexta and their possible roles in the expression regulation of immunity-related genesq Xiufeng Zhang a, Yun Zheng b, Guru Jagadeeswaran c, Ren Ren d, Ramanjulu Sunkar c, Haobo Jiang a, * a Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA b School of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China c Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA d School of Life Sciences, Fudan University, Shanghai 200433, China a r t i c l e i n f o a b s t r a c t Article history: The tobacco hornworm Manduca sexta has served as a model for insect biochemical and physiological Received 8 December 2013 research for decades. However, knowledge of the posttranscriptional regulation of gene expression by Received in revised form microRNAs is still rudimentary in this species. Our previous study (Zhang et al., 2012) identified 163 22 January 2014 conserved and 13 novel microRNAs in M. sexta, most of which were present at low levels in pupae. To Accepted 26 January 2014 identify additional M. sexta microRNAs and more importantly to examine their possible roles in the