Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China》.pdfVIP

Soil Biology Biochemistry 90 (2015) 42e51 Contents lists available at ScienceDirect Soil Biology Biochemistry journal homepage: /locate/soilbio Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China Jing Zhou a, b, Dawei Guan a, Baoku Zhou c, Baisuo Zhao d, Mingchao Ma a, d, Jie Qin a, Xin Jiang a, d, Sanfeng Chen b, Fengming Cao a, d, Delong Shen d, Jun Li a, d, * a Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China b College of Biological Sciences, China Agricultural University, Beijing 100094, PR China c The Institute of Soil Fertility and Environmental Sources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China d Laboratory of Quality Safety Risk Assessment for Microbial Products (Beijing), Ministry of Agriculture, Beijing 100081, PR China a r t i c l e i n f o a b s t r a c t Article history: Although the effects of chemical fertilization management on microbial communities in soils have been Received 17 December 2014 well studied, few studies have examined such impacts of long-term chemical fertilizations on the mi- Received in revised form crobial community in black soils common to northeast China. We applied high-throughput pyrose- 29 May 2015 quencing and quantitative PCR of the 16S rRNA gene to investigate bacterial communities in a long-term Accepted 10 July 2015 f